| pubmed-article:18629488 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C0024880 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C1414557 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C1414301 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:18629488 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:18629488 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:18629488 | pubmed:dateCreated | 2008-9-3 | lld:pubmed |
| pubmed-article:18629488 | pubmed:abstractText | The high-affinity receptor for immunoglobulin E (IgE), FcepsilonRI, is specifically expressed in mast cells and basophils and plays a key role in IgE-mediated allergic reactions. The transcription factor Elf-1 has been previously identified to bind to the promoter of the human FcepsilonRI alpha-chain, which is essential for the function and expression of FcepsilonRI. In the present study, Elf-1 siRNA was conducted to evaluate the effects of Elf-1 on FcepsilonRI alpha-chain expression in the primary mouse mast cells, bone marrow-derived mast cells (BMMC). Introduction of Elf-1 siRNA effectively reduced expression levels of Elf-1 mRNA and protein in BMMC. Transient reporter assay showed that the knockdown of Elf-1 by siRNA resulted in increased FcepsilonRI alpha-chain promoter activity, while overexpression of Elf-1 suppressed alpha-chain promoter activity in BMMC. Elf-1 siRNA-treated BMMC exhibited marked upregulation of FcepsilonRI alpha-chain transcription, whereas beta-chain mRNA was not affected by Elf-1 siRNA. Chromatin immunoprecipitation assay showed that the amount of transcription factor PU.1, recognizing the cis-element close to the Elf-1-site on the FcepsilonRI alpha-chain promoter, was significantly increased by introduction of Elf-1 siRNA. These results indicate that Elf-1 negatively regulates FcepsilonRI alpha-chain expression by suppressing PU.1-mediated transcription of the alpha-chain in BMMC. | lld:pubmed |
| pubmed-article:18629488 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18629488 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18629488 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18629488 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:18629488 | pubmed:issn | 0093-7711 | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:OkumuraKoK | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:OgawaHideokiH | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:NishiyamaChih... | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:WangQing-HuiQ... | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:NakanoNobuhir... | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:ShimokawaNaom... | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:KanadaShunsuk... | lld:pubmed |
| pubmed-article:18629488 | pubmed:author | pubmed-author:HaraMutsukoM | lld:pubmed |
| pubmed-article:18629488 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18629488 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:18629488 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18629488 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18629488 | pubmed:pagination | 557-63 | lld:pubmed |
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| pubmed-article:18629488 | pubmed:meshHeading | pubmed-meshheading:18629488... | lld:pubmed |
| pubmed-article:18629488 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18629488 | pubmed:articleTitle | Suppressive effect of Elf-1 on FcepsilonRI alpha-chain expression in primary mast cells. | lld:pubmed |
| pubmed-article:18629488 | pubmed:affiliation | Atopy Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. | lld:pubmed |
| pubmed-article:18629488 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18629488 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
