Source:http://linkedlifedata.com/resource/pubmed/id/18629488
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2008-9-3
|
| pubmed:abstractText |
The high-affinity receptor for immunoglobulin E (IgE), FcepsilonRI, is specifically expressed in mast cells and basophils and plays a key role in IgE-mediated allergic reactions. The transcription factor Elf-1 has been previously identified to bind to the promoter of the human FcepsilonRI alpha-chain, which is essential for the function and expression of FcepsilonRI. In the present study, Elf-1 siRNA was conducted to evaluate the effects of Elf-1 on FcepsilonRI alpha-chain expression in the primary mouse mast cells, bone marrow-derived mast cells (BMMC). Introduction of Elf-1 siRNA effectively reduced expression levels of Elf-1 mRNA and protein in BMMC. Transient reporter assay showed that the knockdown of Elf-1 by siRNA resulted in increased FcepsilonRI alpha-chain promoter activity, while overexpression of Elf-1 suppressed alpha-chain promoter activity in BMMC. Elf-1 siRNA-treated BMMC exhibited marked upregulation of FcepsilonRI alpha-chain transcription, whereas beta-chain mRNA was not affected by Elf-1 siRNA. Chromatin immunoprecipitation assay showed that the amount of transcription factor PU.1, recognizing the cis-element close to the Elf-1-site on the FcepsilonRI alpha-chain promoter, was significantly increased by introduction of Elf-1 siRNA. These results indicate that Elf-1 negatively regulates FcepsilonRI alpha-chain expression by suppressing PU.1-mediated transcription of the alpha-chain in BMMC.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ephrin-A2,
http://linkedlifedata.com/resource/pubmed/chemical/FCER1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein Spi-1
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
0093-7711
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
60
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
557-63
|
| pubmed:meshHeading |
pubmed-meshheading:18629488-Animals,
pubmed-meshheading:18629488-Blotting, Western,
pubmed-meshheading:18629488-Bone Marrow,
pubmed-meshheading:18629488-Chromatin Immunoprecipitation,
pubmed-meshheading:18629488-Ephrin-A2,
pubmed-meshheading:18629488-Gene Expression Regulation,
pubmed-meshheading:18629488-Humans,
pubmed-meshheading:18629488-Luciferases,
pubmed-meshheading:18629488-Mast Cells,
pubmed-meshheading:18629488-Mice,
pubmed-meshheading:18629488-Promoter Regions, Genetic,
pubmed-meshheading:18629488-Proto-Oncogene Proteins,
pubmed-meshheading:18629488-RNA, Messenger,
pubmed-meshheading:18629488-RNA, Small Interfering,
pubmed-meshheading:18629488-Receptors, IgE,
pubmed-meshheading:18629488-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18629488-Trans-Activators,
pubmed-meshheading:18629488-Transcription, Genetic,
pubmed-meshheading:18629488-Up-Regulation
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| pubmed:year |
2008
|
| pubmed:articleTitle |
Suppressive effect of Elf-1 on FcepsilonRI alpha-chain expression in primary mast cells.
|
| pubmed:affiliation |
Atopy Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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