Linked Life Data

18628491

Source:http://linkedlifedata.com/resource/pubmed/id/18628491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2008-7-16
pubmed:abstractText
A mini-Tn5lacZ1 transposon insertion in a gene encoding an orthologue of the Lon protease conferred a hyper-swarming phenotype on Proteus mirabilis. The lon mutation increased the accumulation of mRNA for representative class 1 (flhDC), class 2 (fliA) and class 3 (flaA) genes during swarmer cell differentiation. In addition, the stability of the FlhD protein was fourfold higher in the lon : mini-Tn5lacZ1 background. Expression of a single-copy lon : lacZ fusion increased during the swarming cycle and reached peak levels of expression at a point just after swarmer cell differentiation had initiated. In liquid media, a condition normally non-permissive for swarming, the lon : : mini-Tn5lacZ1 insertion resulted in motile, highly elongated cells that overexpressed flagellin. Finally, the lon : : mini-Tn5lacZ1 mutation was shown to result in increased expression of the hpmBA and zapA virulence genes during swarmer cell differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-2615
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
931-7
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The Lon protease regulates swarming motility and virulence gene expression in Proteus mirabilis.
pubmed:affiliation
Research Service, Veterans Affairs Medical Center, Decatur, GA, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.