Linked Life Data

18623243

Source:http://linkedlifedata.com/resource/pubmed/id/18623243

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-9-11
pubmed:abstractText
In vertebrate rod photoreceptor cells, arrestin and the visual G-protein transducin move between the inner segment and outer segment in response to changes in light. This stimulus dependent translocation of signalling molecules is assumed to participate in long term light adaptation of photoreceptors. So far the cellular basis for the transport mechanisms underlying these intracellular movements remains largely elusive. Here we investigated the dependency of these movements on actin filaments and the microtubule cytoskeleton of photoreceptor cells. Co-cultures of mouse retina and retinal pigment epithelium were incubated with drugs stabilizing and destabilizing the cytoskeleton. The actin and microtubule cytoskeleton and the light dependent distribution of signaling molecules were subsequently analyzed by light and electron microscopy. The application of cytoskeletal drugs differentially affected the cytoskeleton in photoreceptor compartments. During dark adaptation the depolymerization of microtubules as well as actin filaments disrupted the translocation of arrestin and transducin in rod photoreceptor cells. During light adaptation only the delivery of arrestin within the outer segment was impaired after destabilization of microtubules. Movements of transducin and arrestin required intact cytoskeletal elements in dark adapting cells. However, diffusion might be sufficient for the fast molecular movements observed as cells adapt to light. These findings indicate that different molecular translocation mechanisms are responsible for the dark and light associated translocations of arrestin and transducin in rod photoreceptor cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1097-0169
pubmed:author
pubmed:copyrightInfo
Copyright 2008 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
785-800
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:18623243-Actin Cytoskeleton, pubmed-meshheading:18623243-Actins, pubmed-meshheading:18623243-Animals, pubmed-meshheading:18623243-Arrestin, pubmed-meshheading:18623243-Cell Migration Assays, pubmed-meshheading:18623243-Cytochalasin D, pubmed-meshheading:18623243-Cytoskeleton, pubmed-meshheading:18623243-Dark Adaptation, pubmed-meshheading:18623243-Darkness, pubmed-meshheading:18623243-Heterocyclic Compounds with 4 or More Rings, pubmed-meshheading:18623243-Homozygote, pubmed-meshheading:18623243-Light, pubmed-meshheading:18623243-Mice, pubmed-meshheading:18623243-Mice, Inbred C57BL, pubmed-meshheading:18623243-Mice, Knockout, pubmed-meshheading:18623243-Microscopy, Fluorescence, pubmed-meshheading:18623243-Microscopy, Immunoelectron, pubmed-meshheading:18623243-Microtubules, pubmed-meshheading:18623243-Paclitaxel, pubmed-meshheading:18623243-Phalloidine, pubmed-meshheading:18623243-Protein Transport, pubmed-meshheading:18623243-Retina, pubmed-meshheading:18623243-Retinal Rod Photoreceptor Cells, pubmed-meshheading:18623243-Rod Cell Outer Segment, pubmed-meshheading:18623243-Signal Transduction, pubmed-meshheading:18623243-Thiabendazole, pubmed-meshheading:18623243-Transducin, pubmed-meshheading:18623243-Vision, Ocular
pubmed:year
2008
pubmed:articleTitle
The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton.
pubmed:affiliation
Department of Cell and Matrix Biology, Institute of Zoology, Johannes Gutenberg University of Mainz, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't