Linked Life Data

18623042

Source:http://linkedlifedata.com/resource/pubmed/id/18623042

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-7-21
pubmed:abstractText
The present study sought to establish a standard in vivo imaging procedure for mouse kidney anatomy evaluation using contrast-enhanced high-resolution X-ray microtomography (micro-CT). Micro-CT estimation of kidney volume was compared with ex vivo measurement by micro-CT and water displacement. Control values were obtained in four strains (BALB/c, C3H/HeN, 129/Sv and C57BL/6J) of healthy male and female mice aged 22 +/- 2 weeks. An excellent correlation was found between in vivo and ex vivo kidney volumes (n = 26 mice; 52 kidneys; r = 0.96). In vivo measurement systematically overestimated ex vivo kidney volume by 28 +/- 4%, while there was no significant difference between the ex vivo micro-CT value and the true kidney volume on water displacement (2.3 +/- 2.1%). In vivo kidney volume also correlated strongly with kidney weight and in vivo kidney length (n = 52 mice; 104 kidneys; r = 0.84, r = 0.92 respectively). Differences between strains were observed for kidney volume when comparing either kidney volume or kidney weight to body weight. In conclusion, this study demonstrated that contrast-enhanced micro-CT enables accurate in vivo measurement of kidney volume, length and thickness in mice. Reference parameters are reported for four strains. The technique provides a useful follow-up research tool for mouse phenotyping and renal disease studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1555-4317
pubmed:author
pubmed:copyrightInfo
Copyright 2008 John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
120-6
pubmed:dateRevised
2010-10-29
pubmed:meshHeading
pubmed:articleTitle
Kidney volume quantification using contrast-enhanced in vivo X-ray micro-CT in mice.
pubmed:affiliation
ANIMAGE, Rhône-Alpes GENOPOLE, Université de Lyon, Bât. CERMEP-59, Boulevard Pinel, 69677 Bron Cedex, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Validation Studies