| pubmed-article:18620062 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C0035366 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C1413269 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C0162768 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C0599566 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C1517050 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:18620062 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:18620062 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18620062 | pubmed:dateCreated | 2008-9-1 | lld:pubmed |
| pubmed-article:18620062 | pubmed:abstractText | Osteoblast cadherin (OB-cadherin, also known as cadherin-11) is a Ca(2+)-dependent homophilic cell adhesion molecule that is expressed mainly in osteoblasts. OB-cadherin is expressed in prostate cancer and may be involved in the homing of metastatic prostate cancer cells to bone. The extracellular domain of OB-cadherin may be used to inhibit the adhesion between prostate cancer cells and osteoblasts. In this report, we describe the expression of the extracellular domain of OB-cadherin as an Fc fusion protein (OB-CAD-Fc) in human embryonic kidney 293FT cells using a bicistronic retroviral vector. Coexpression of GFP and OB-CAD-Fc through the bicistronic vector permitted enrichment of OB-CAD-Fc-expressing cells by fluorescence-activated cell sorting. Recombinant OB-CAD-Fc proteins were secreted into cell medium, and about 0.85 mg of purified OB-CAD-Fc protein was purified from 1l of the conditioned medium using immobilized protein A-affinity chromatography. The purified OB-CAD-Fc was biologically active because it supported the adhesion of PC3 cells and L cells transduced with OB-cadherin. The availability of OB-CAD-Fc offers opportunities to test whether OB-CAD-Fc can be used to inhibit OB-cadherin-mediated prostate cancer bone metastasis in vivo or to generate antibodies for inhibiting the adhesion between prostate cancer cells and osteoblasts. | lld:pubmed |
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| pubmed-article:18620062 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18620062 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18620062 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18620062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18620062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18620062 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18620062 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:18620062 | pubmed:issn | 1096-0279 | lld:pubmed |
| pubmed-article:18620062 | pubmed:author | pubmed-author:HuMickey... | lld:pubmed |
| pubmed-article:18620062 | pubmed:author | pubmed-author:KiddF SFS | lld:pubmed |
| pubmed-article:18620062 | pubmed:author | pubmed-author:LinSue-HwaSH | lld:pubmed |
| pubmed-article:18620062 | pubmed:author | pubmed-author:LeeYu-ChenYC | lld:pubmed |
| pubmed-article:18620062 | pubmed:author | pubmed-author:LiraCristina... | lld:pubmed |
| pubmed-article:18620062 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18620062 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:18620062 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18620062 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18620062 | pubmed:pagination | 220-6 | lld:pubmed |
| pubmed-article:18620062 | pubmed:dateRevised | 2011-9-26 | lld:pubmed |
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| pubmed-article:18620062 | pubmed:meshHeading | pubmed-meshheading:18620062... | lld:pubmed |
| pubmed-article:18620062 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18620062 | pubmed:articleTitle | Expression of the extracellular domain of OB-cadherin as an Fc fusion protein using bicistronic retroviral expression vector. | lld:pubmed |
| pubmed-article:18620062 | pubmed:affiliation | Department of Molecular Pathology, The University of Texas M.D. Anderson Cancer Center, Unit 89, 1515 Holcombe Boulevard, Houston, TX 77030, USA. liracbb@gmail.com | lld:pubmed |
| pubmed-article:18620062 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18620062 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:18620062 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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