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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-9-1
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pubmed:abstractText |
Osteoblast cadherin (OB-cadherin, also known as cadherin-11) is a Ca(2+)-dependent homophilic cell adhesion molecule that is expressed mainly in osteoblasts. OB-cadherin is expressed in prostate cancer and may be involved in the homing of metastatic prostate cancer cells to bone. The extracellular domain of OB-cadherin may be used to inhibit the adhesion between prostate cancer cells and osteoblasts. In this report, we describe the expression of the extracellular domain of OB-cadherin as an Fc fusion protein (OB-CAD-Fc) in human embryonic kidney 293FT cells using a bicistronic retroviral vector. Coexpression of GFP and OB-CAD-Fc through the bicistronic vector permitted enrichment of OB-CAD-Fc-expressing cells by fluorescence-activated cell sorting. Recombinant OB-CAD-Fc proteins were secreted into cell medium, and about 0.85 mg of purified OB-CAD-Fc protein was purified from 1l of the conditioned medium using immobilized protein A-affinity chromatography. The purified OB-CAD-Fc was biologically active because it supported the adhesion of PC3 cells and L cells transduced with OB-cadherin. The availability of OB-CAD-Fc offers opportunities to test whether OB-CAD-Fc can be used to inhibit OB-cadherin-mediated prostate cancer bone metastasis in vivo or to generate antibodies for inhibiting the adhesion between prostate cancer cells and osteoblasts.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-10202138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-10835267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-10910081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-11204426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-11449003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-11962605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-12770907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-15265297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-15546992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-16564015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-16989088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-17347145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-18393855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-1970124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-2006419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-2174810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-3048970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-7750650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-7885471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-8163513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-8598933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-8853994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18620062-9821270
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1096-0279
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
220-6
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:18620062-Blotting, Western,
pubmed-meshheading:18620062-Cadherins,
pubmed-meshheading:18620062-Cell Adhesion,
pubmed-meshheading:18620062-Cell Line,
pubmed-meshheading:18620062-Chromatography, Affinity,
pubmed-meshheading:18620062-Culture Media, Conditioned,
pubmed-meshheading:18620062-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18620062-Genetic Vectors,
pubmed-meshheading:18620062-Humans,
pubmed-meshheading:18620062-Immunoglobulin Fc Fragments,
pubmed-meshheading:18620062-Kidney,
pubmed-meshheading:18620062-Protein Structure, Tertiary,
pubmed-meshheading:18620062-Recombinant Fusion Proteins,
pubmed-meshheading:18620062-Retroviridae,
pubmed-meshheading:18620062-Time Factors
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pubmed:year |
2008
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pubmed:articleTitle |
Expression of the extracellular domain of OB-cadherin as an Fc fusion protein using bicistronic retroviral expression vector.
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pubmed:affiliation |
Department of Molecular Pathology, The University of Texas M.D. Anderson Cancer Center, Unit 89, 1515 Holcombe Boulevard, Houston, TX 77030, USA. liracbb@gmail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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