Source:http://linkedlifedata.com/resource/pubmed/id/18619551
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2008-8-25
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| pubmed:abstractText |
We explored molecular and morphological alteration in gill mitochondria-rich (MR) cells of Mozambique tilapia, Oreochromis mossambicus, acclimated to deionized freshwater (DFW), freshwater (FW), 1/3-diluted seawater (1/3 SW) and seawater (SW). Scanning electron microscopic observations revealed that the apical membrane of MR cells appeared as a flat or slightly projecting disk in DFW and FW, being larger in DFW than in FW. In contrast, the apical membrane typically formed a pit structure in 1/3 SW and SW. The mRNA expression levels of Na(+)/H(+) exchanger-3 (NHE3) and Na(+)/Cl(-) cotransporter (NCC) in the gills were increased with decreasing environmental salinity, whereas Na(+)/K(+)/2Cl(-) cotransporter-1a (NKCC1a) expression was upregulated by increasing salinity. Immunofluorescence staining showed that the MR cell population of DFW- and FW-acclimated tilapia consisted mostly of MR cells with apical NHE3 and those with apical-NCC; MR cells with basolateral NKCC1a dominated in SW-acclimated tilapia. These results indicated that apical-NHE3 and apical-NCC MR cells were ion-absorbing cells, and that basolateral-NKCC1a MR cells were ion-secreting cells. In fish acclimated to 1/3 SW, both ion-absorbing and secreting cells existed in the gills, suggesting that fish in near-isotonic water were equipped with mechanisms of both hyper- and hypoosmoregulation to prepare for environmental salinity changes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ion Pumps,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Chloride Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-hydrogen exchanger 3
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1531-4332
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
151
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
151-8
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| pubmed:meshHeading |
pubmed-meshheading:18619551-Acclimatization,
pubmed-meshheading:18619551-Animals,
pubmed-meshheading:18619551-Base Sequence,
pubmed-meshheading:18619551-Fluorescent Antibody Technique,
pubmed-meshheading:18619551-Fresh Water,
pubmed-meshheading:18619551-Gene Expression Regulation,
pubmed-meshheading:18619551-Gills,
pubmed-meshheading:18619551-Ion Pumps,
pubmed-meshheading:18619551-Microscopy, Electron, Scanning,
pubmed-meshheading:18619551-Mitochondria,
pubmed-meshheading:18619551-RNA, Messenger,
pubmed-meshheading:18619551-Seawater,
pubmed-meshheading:18619551-Sodium Chloride,
pubmed-meshheading:18619551-Sodium Chloride Symporters,
pubmed-meshheading:18619551-Sodium-Hydrogen Antiporter,
pubmed-meshheading:18619551-Sodium-Potassium-Chloride Symporters,
pubmed-meshheading:18619551-Staining and Labeling,
pubmed-meshheading:18619551-Tilapia
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| pubmed:year |
2008
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| pubmed:articleTitle |
Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinities.
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| pubmed:affiliation |
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan. niida@marine.fs.a.u-tokyo.ac.jp
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| pubmed:publicationType |
Journal Article
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