Linked Life Data

18619488

Source:http://linkedlifedata.com/resource/pubmed/id/18619488

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-11-25
pubmed:abstractText
The ability of docosahexaenoic acid (DHA) to modulate methylmercury (MeHg)-induced neurotoxicity was investigated in primary astrocytes and neurons from the cerebellum. Gas chromatography measurements indicated increased DHA content in both cell types after 24h supplementation. After individual or combined treatment with MeHg (10microM) and DHA (30 and 90microM), the cell-associated MeHg measurements were done using (14)C-labelled MeHg. In addition, mitochondrial activity was evaluated by MTT reduction, glutathione (GSH) content was measured with the fluorescent indicator monochlorobimane (MCB) and reactive oxygen species (ROS) were detected with the fluorescent indicator-chloro methyl derivative of di-chloro di-hydro fluorescein diacetate (CMH(2)DCFDA). For all the tested treatments, i.e. DHA, MeHg or DHA+MeHg treatment, the neurons differed significantly (p<0.001) from astrocytes exhibiting increased ROS production and decreased MTT activity. After MeHg and 30microM DHA treatment there were no changes in MTT or GSH content but significant decrease (p<0.001) in ROS was observed in both the cell types when compared to MeHg alone. The cell-associated MeHg measurements indicated reduced MeHg-accumulation in both cell types (p<0.05) upon 30microM DHA exposure. Taken together, this study, for the first time establishes that DHA pretreatment effectively reduces cell-associated MeHg and prooxidant response from MeHg in both cerebellar astrocytes and neurons and thus supports the hypothesis that fish-derived nutrients offer possible neuroprotection from MeHg.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0161-813X
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
978-87
pubmed:meshHeading
pubmed-meshheading:18619488-Analysis of Variance, pubmed-meshheading:18619488-Animals, pubmed-meshheading:18619488-Animals, Newborn, pubmed-meshheading:18619488-Astrocytes, pubmed-meshheading:18619488-Carbon Isotopes, pubmed-meshheading:18619488-Cells, Cultured, pubmed-meshheading:18619488-Cerebellum, pubmed-meshheading:18619488-Docosahexaenoic Acids, pubmed-meshheading:18619488-Dose-Response Relationship, Drug, pubmed-meshheading:18619488-Drug Interactions, pubmed-meshheading:18619488-Fatty Acids, pubmed-meshheading:18619488-Fluoresceins, pubmed-meshheading:18619488-Glutathione, pubmed-meshheading:18619488-Methylmercury Compounds, pubmed-meshheading:18619488-Mice, pubmed-meshheading:18619488-Neurons, pubmed-meshheading:18619488-Reactive Oxygen Species, pubmed-meshheading:18619488-Tetrazolium Salts, pubmed-meshheading:18619488-Thiazoles
pubmed:year
2008
pubmed:articleTitle
Docosahexaenoic acid may act as a neuroprotector for methylmercury-induced neurotoxicity in primary neural cell cultures.
pubmed:affiliation
Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Olav Kyrresgt. 3, N-7489 Trondheim, Norway. parvinder.kaur@ntnu.no
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural