Linked Life Data

18617029

Source:http://linkedlifedata.com/resource/pubmed/id/18617029

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-7-11
pubmed:abstractText
Three-dimensional structure of cells and organelles examined with the power of resolution of electron microscopy (EM) including EM tomography represents the average view of cell processes. More precise and detailed analysis is limited by the significant variations in the structure of cells in temporal dynamics of cellular events. Therefore EM cannot identify rare and fast events that could be extremely important for understanding molecular mechanisms underlying these cellular processes. Observation of living cells under EM is still impossible. In contrast, observations of cellular dynamics with the help of laser scanning confocal microscopes or light digitalized microscopes became an indispensable tool of cell biology. However, the resolution of even confocal microscope is limited to the half of the light wave. Therefore, in studies of dynamic cellular processes, it would be ideal to be able to combine the capability of in vivo fluorescence video microscopy with the EM. This chapter describes this technique with details and useful tricks, including the way to localize the same cell after its transfection with a protein fused with a fluorescent tag, examination under the microscope in living condition, fixation, immunolabeling, embedding, serial sectioning from the first section, and observation under EM. We also illustrate here the kinds of questions that the CVLEM approach was designed to address, as well as the particular know-how that is important for the successful application of this technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0091-679X
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-95
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Combined video fluorescence and 3D electron microscopy.
pubmed:affiliation
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Chieti, Italy.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't