|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0002771,
umls-concept:C0007634,
umls-concept:C0023951,
umls-concept:C0034693,
umls-concept:C0205096,
umls-concept:C0231491,
umls-concept:C0332281,
umls-concept:C0392747,
umls-concept:C0441655,
umls-concept:C0443172,
umls-concept:C1280500,
umls-concept:C1305694,
umls-concept:C1414512,
umls-concept:C1427405,
umls-concept:C1564816,
umls-concept:C1999216
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2008-10-28
|
|
pubmed:abstractText |
We evaluated the effects of intra-periaqueductal grey (PAG) N-arachidonoyl-serotonin (AA-5-HT), a compound with a "dual" ability to inhibit the fatty acid amide hydrolase (FAAH) and to antagonize transient receptor vanilloid type 1 (TRPV1) receptors, on endocannabinoid levels, rostral ventromedial medulla (RVM) ON and OFF cell activities, thermal nociception (tail flick in anaesthetized rats) and formalin-induced nocifensive responses in awake rats. AA-5-HT increased endocannabinoid levels in the PAG and induced analgesia. Paradoxically, it also depressed the RVM OFF cell, as well as the ON cell activities. The effect of AA-5-HT was mimicked by co-injecting the selective FAAH inhibitor URB597 and the selective TRPV1 antagonist I-RTX into the PAG, which also induced analgesia and inhibition of ON and OFF cell ongoing activities. The recruitment of "alternative" pathways, such as PAG-locus coeruleus (LC)-spinal cord might be responsible for AA-5-HT effect since we found evidence that (i) intra-PAG AA-5-HT increased LC neuron firing activities, and (ii) intrathecal phentolamine or ketanserin prevented the analgesic effect of AA-5-HT. Moreover, intra-PAG AA-5-HT prevented the changes in the ON and OFF cells firing activity induced by intra-paw formalin, and it inverted the formalin-induced increase in LC adrenergic cell activity. All AA-5-HT effects were antagonized by cannabinoid CB1 and TRPV1 receptor antagonists thus suggesting that co-localization of these receptors in the PAG can be an appropriate neural substrate for AA-5-HT-induced analgesia.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Amidohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Formaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV1 receptor,
http://linkedlifedata.com/resource/pubmed/chemical/arachidonoylserotonin,
http://linkedlifedata.com/resource/pubmed/chemical/fatty-acid amide hydrolase,
http://linkedlifedata.com/resource/pubmed/chemical/oleoylethanolamide,
http://linkedlifedata.com/resource/pubmed/chemical/palmidrol
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0028-3908
|
|
pubmed:author |
pubmed-author:CristinoLuigiaL,
pubmed-author:De PetrocellisLucianoL,
pubmed-author:Di MarzoVincenzoV,
pubmed-author:Endocannabinoid Research Group,
pubmed-author:GuidaFrancescaF,
pubmed-author:LuongoLivioL,
pubmed-author:MaioneSabatinoS,
pubmed-author:MarabeseIdaI,
pubmed-author:MigliozziAnnaluciaA,
pubmed-author:PalazzoEnzaE,
pubmed-author:PetrosinoStefaniaS,
pubmed-author:RossiFrancescaF,
pubmed-author:StarowiczKatarzynaK,
pubmed-author:de NovellisVitoV
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
55
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1105-13
|
|
pubmed:meshHeading |
pubmed-meshheading:18616956-Adrenergic Antagonists,
pubmed-meshheading:18616956-Amidohydrolases,
pubmed-meshheading:18616956-Analgesics,
pubmed-meshheading:18616956-Animals,
pubmed-meshheading:18616956-Arachidonic Acids,
pubmed-meshheading:18616956-Electrophysiology,
pubmed-meshheading:18616956-Extracellular Space,
pubmed-meshheading:18616956-Formaldehyde,
pubmed-meshheading:18616956-Locus Coeruleus,
pubmed-meshheading:18616956-Male,
pubmed-meshheading:18616956-Medulla Oblongata,
pubmed-meshheading:18616956-Microinjections,
pubmed-meshheading:18616956-Oleic Acids,
pubmed-meshheading:18616956-Pain Measurement,
pubmed-meshheading:18616956-Palmitic Acids,
pubmed-meshheading:18616956-Rats,
pubmed-meshheading:18616956-Reaction Time,
pubmed-meshheading:18616956-Serotonin,
pubmed-meshheading:18616956-Serotonin Antagonists,
pubmed-meshheading:18616956-TRPV Cation Channels
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
The analgesic effect of N-arachidonoyl-serotonin, a FAAH inhibitor and TRPV1 receptor antagonist, associated with changes in rostral ventromedial medulla and locus coeruleus cell activity in rats.
|
|
pubmed:affiliation |
Department of Experimental Medicine, Section of Pharmacology L. Donatelli, Second University of Naples, Naples, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
