Linked Life Data

18613648

Source:http://linkedlifedata.com/resource/pubmed/id/18613648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2008-8-15
pubmed:abstractText
In this article, we report a simple approach for the selective sensing of homocysteine thiolactone (HTL) using fluorosurfactant (FSN)-capped gold nanoparticles (AuNPs) as aminothiol removers and as sensors. We have shown that HTL did not bind to the surface of the FSN-AuNPs in the pH range of 4.0-10.0. In contrast, under these pH conditions, the FSN-AuNPs are aggregated upon the addition of homocysteine (HCys) and cysteine (Cys). On the basis of this feature, we have demonstrated that FSN-AuNPs are effective sorbent materials for HCys and Cys, but not for HTL. It is found that the FSN-AuNPs can remove of >98% of HCys and >99% of Cys from an aqueous solution. Thus, after the centrifugation of a solution containing AuNPs, HTL, and other aminothiols, only HTL remains in the supernatant. When NaOH is added to the supernatant, HTL is hydrolyzed to HCys, leading to the aggregation of the FSN-AuNPs. As a result, the selectivity of the probe is significantly higher for HTL in aqueous solutions than for other aminthiols. The sensitivity of FSN-AuNPs toward HTL can be further improved by optimizing the AuNP concentrations. Under optimum conditions, the lowest detectable concentration of HTL through this approach is 100 nM. We have validated the applicability of our method through the analyses of HTL in urine samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1520-6882
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6345-50
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Role of fluorosurfactant-modified gold nanoparticles in selective detection of homocysteine thiolactone: remover and sensor.
pubmed:affiliation
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't