Guidelines for assessing the potential allergenicity of genetically modified (GM) organisms recommend testing the digestibility of the introduced protein by pepsin. Previous studies detailed the digestion procedure but have not described a simple objective measurement of the extent of digestion nor evaluated the impact of variation in pepsin activity.
Food Allergy Research and Resource Program (FARRP), Department of Food Science & Technology, University of Nebraska-Lincoln, 143 Food Industry Complex, Lincoln, NE 68583-0955, USA.