Linked Life Data

18610633

Source:http://linkedlifedata.com/resource/pubmed/id/18610633

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-7-9
pubmed:abstractText
To screen the genes associated with esophageal cancer, a cDNA microarray technique was established and used for the analysis of the gene expression profile in human esophageal cancer cell line ECa109. The results showed that 107 (12.08%) genes differentially expressed among 886 target genes were identified between ECa109 cell line and normal human esophageal epithelial cells (HEEC), of which 51 (5.76%) were up-regulated and 56 (6.32%) down-regulated. Two genes were validated by quantitative RT-PCR (Q-RT-PCR) and the results were identical. The RNA amplification technique based-T7 RNA polymerase was established. The gene expression profile revealed better consistency between the amplified samples and those without amplification by T7 RNA polymerase, which provides a method for studying the profile of minute quantities of tumor cells in primary esophageal cancers. And the preliminary study on differential expression gene profile also enables us to have an understanding of the pathogenesis and pathomechanism of esophageal cancer.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1001-5515
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
413-8
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
[Establishment of cDNA microarray technology and analysis of gene expression profiles in human esophageal cancer cell line ECa109].
pubmed:affiliation
Department of Pathophysiology, Zhengzhou University of Medical Sciences, Zhengzhou 450052, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't