Source:http://linkedlifedata.com/resource/pubmed/id/18609622
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2008-7-8
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| pubmed:abstractText |
Fed-batch techniques were employed to obtain high cell density cultures (92-100 g DCW/L) of Escherichia coli strain X90 producing a recombinant serine protease, rat anionic trypsin, secreted to the periplasm. The specific growth rate was controlled to minimize growth-inhibiting acetate formation by utilizing an exponential feeding profile determined from mass balance equation. The volumetric yield of recombinant rat anionic trypsin was 56 mg/L, and the final cell density was 92 g DCW/L when the culture was induced in the late logarithmic phase. However, when the culture was induced in the early logarithmic phase, the volumetric yield was 13 mg/L and the final cell density was 14 g DCW/L. Thus, the induction timing is shown to have a significant effect on the final cell density as well as the overall volumetric yield of the recombinant protease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0006-3592
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| pubmed:author | |
| pubmed:copyrightInfo |
(c) 1993 Wiley & Sons, Inc.
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| pubmed:issnType |
Print
|
| pubmed:day |
5
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| pubmed:volume |
41
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
781-90
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| pubmed:year |
1993
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| pubmed:articleTitle |
Recombinant trypsin production in high cell density fed-batch cultures in Escherichia coli.
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| pubmed:affiliation |
Department of Chemical Engineering, University of California at Berkeley, Berkeley, California 94702, USA.
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| pubmed:publicationType |
Journal Article
|