|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1991-9-5
|
|
pubmed:abstractText |
The effects of prolactin (PRL) on A10 (aortic smooth muscle) cell proliferation were examined by measuring both [3H]thymidine incorporation and increases in cell number. PRL induced a significant proliferative response from 10(-11) to 10(-7) M, with optimal activity at 10(-10) M. PRL also enhanced platelet-derived growth factor (PDGF)-induced proliferation. The possibility that PRL induces proliferation through a protein kinase C (PKC)-mediated mechanism was also examined. PRL caused activation of PKC from 10(-12) to 10(-8) M. Antiserum to PRL, a monoclonal antibody directed against the PRL receptor and the immunosuppressive agent cyclosporine A, were able to inhibit PRL-induced proliferation and activation of PKC. The PKC inhibitors, staurosporine, sphingosine, and 1-(-5-iso-quinoline-sulfonyl)-2-methylpiperazine (H-7) also antagonized both proliferation and PKC activation. These data strongly suggest that PRL-induced A10 cell proliferation is mediated through the PKC pathway and that this may play a role in vascular smooth muscle cell hyperplasia, characteristic of the pathogenesis of cardiovascular diseases such as hypertension and atherosclerosis.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp...,
http://linkedlifedata.com/resource/pubmed/chemical/Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclosporins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingosine,
http://linkedlifedata.com/resource/pubmed/chemical/Staurosporine,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0021-9541
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
148
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
133-8
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1860893-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine,
pubmed-meshheading:1860893-Alkaloids,
pubmed-meshheading:1860893-Animals,
pubmed-meshheading:1860893-Cell Division,
pubmed-meshheading:1860893-Cells, Cultured,
pubmed-meshheading:1860893-Cyclosporins,
pubmed-meshheading:1860893-DNA,
pubmed-meshheading:1860893-Enzyme Activation,
pubmed-meshheading:1860893-Isoquinolines,
pubmed-meshheading:1860893-Muscle, Smooth, Vascular,
pubmed-meshheading:1860893-Piperazines,
pubmed-meshheading:1860893-Platelet-Derived Growth Factor,
pubmed-meshheading:1860893-Prolactin,
pubmed-meshheading:1860893-Protein Kinase C,
pubmed-meshheading:1860893-Receptors, Prolactin,
pubmed-meshheading:1860893-Sphingosine,
pubmed-meshheading:1860893-Staurosporine,
pubmed-meshheading:1860893-Thymidine,
pubmed-meshheading:1860893-Tritium
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Prolactin induces proliferation of vascular smooth muscle cells through a protein kinase C-dependent mechanism.
|
|
pubmed:affiliation |
Department of Pharmacology and Therapeutics, University of South Florida College of Medicine, Tampa.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
