Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1991-9-5
pubmed:databankReference
pubmed:abstractText
Previous studies of the structure and expression of the ribosome-releasing factor (RRF) cistron (frr) have suggested that an efficient promoter region is located in the RRF cistron. We report here on the nucleotide sequence and in vivo function of the RRF promoter. The transcriptional start site was determined by primer extension to be 58 bp upstream of the translational initiation codon of frr. The location of the RRF promoter region was confirmed by means of (i) deletion analysis of the 5' proximal sequences of frr fused to the chloramphenicol acetyltransferase reporter gene, (ii) analysis of RRF produced in vivo from the deletion derivatives of frr cloned into pUC19, and (iii) gel retardation analysis with Escherichia coli RNA polymerase. The -35 and -10 regions were TTacCc and TATAcT, respectively. The strength of the RRF promoter was similar to that of the lac promoter, as determined by in vivo expression of chloramphenicol acetyltransferase activity. However, the RRF promoter was not affected by the intracellular cyclic AMP level despite the presence of a cyclic AMP receptor protein binding site downstream of the RRF promoter.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-1088926, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-15966100, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-199577, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2192653, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2194166, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2204341, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2661533, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2684966, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2840346, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-3032964, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-354508, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-3550697, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-4317918, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-4341750, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-4598299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-4894690, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-4920474, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6087276, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6178738, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6183002, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6186393, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6248238, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6269071, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6297582, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6313226, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6327267, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6327464, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6344016, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6350894, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6372090, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6380764, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-6692468, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-7013987, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-7016861, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-7017348, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-7035835, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-788919, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/1860827-94251
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5181-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Identification of the promoter region of the ribosome-releasing factor cistron (frr).
pubmed:affiliation
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
pubmed:publicationType
Journal Article