Linked Life Data

18606430

Source:http://linkedlifedata.com/resource/pubmed/id/18606430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-8-1
pubmed:abstractText
In the present study, we investigated the role of spinal nitric oxide (NO) in rat pain-related behaviors induced by the venom of scorpion Buthus martensi Karsch (BmK). The results showed that the number of neuronal NO synthase (nNOS) positive neurons significantly increased in superficial (I-II), deep (V-VI) dorsal horn laminae and the ventral gray laminae (VII-X), but not in the nucleus proprius (III and IV) of bilateral L4-L5 lumbar spinal cord after unilateral intraplantar injection of BmK venom from 2h to 7d. This increase on the ipsilateral side to BmK venom injection was always greater than that on the contralateral side. Western blotting analysis confirmed that spinal nNOS expression was significantly up-regulated following BmK venom administration. In addition, intrathecal delivery of N(omega)-nitro-l-arginine methyl ester hydrochloride (l-NAME; a NOS inhibitor) before intraplantar injection of BmK venom by 10 min significantly attenuated spontaneous nociceptive responses and prevented the development of primary thermal hyperalgesia as well as bilateral mechanical hyperalgesia. Intrathecal injection of l-NAME could also partially inhibit BmK venom-induced c-Fos expression in lumbar spinal cord at 2 h. Thus, the results suggest that spinal NO as a critical mediator is involved in various pain-related behaviors and c-Fos expression induced by BmK venom in rats.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
62-71
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Involvement of spinal nitric oxide (NO) in rat pain-related behaviors induced by the venom of scorpion Buthus martensi Karsch.
pubmed:affiliation
Graduate School of the Chinese Academy of Sciences, Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't