Source:http://linkedlifedata.com/resource/pubmed/id/18601204
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2008-7-8
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| pubmed:abstractText |
The suitability of adsorbent polymeric resins, Amberlite XAD-4 and XAD-7 (Rohm and Hass, Inc.), was investigated for the accumulation of sanguinarine from Papaver somniferum cell cultures. The adsorption and desorption of sanguinarine from aqueous solution was most effective with XAD-7. In addition to sanguinarine, the resins were found to absorb growth regulators and vitamins from the culture medium. Growth inhibition was overcome by delaying for approximately 4 days resin addition after cell inoculation in fresh medium. Resin addition (5% wt/vol) to actively growing uneclicited cultures led to increases in sanguinarine production and release of 30% to 40% and 60%, respectively. The addition of resins to elicited cultures led to increases in alkaloid production of up to 50% to 85% with similar increases in alkaloid release as observed for nonelicited cells. Overall yield of sanguinarine increased from 21 mg . g biomass dry weight(-1) (dw) for elicited cultures to more than 39 mg . gdw(-1) when elicitation was combined with resin addition. Higher quantities of resin (10% to 20% wt/vol) increased marginally the release of sanguinarine into the medium, and on the resin, up to 85% of total production. The use of resin appears promising for the development of a bioprocess for sanguinarine production by cultured plant cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0006-3592
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| pubmed:author | |
| pubmed:copyrightInfo |
(c) 1992 John Wiley & Sons, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
20
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| pubmed:volume |
40
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
971-7
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| pubmed:year |
1992
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| pubmed:articleTitle |
Effect of polymeric adsorbents on the production of sanguinarine by Papaver somniferum cell cultures.
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| pubmed:affiliation |
Biotechnology Research Institute, National Research Council of Canada, Montreal, Québec, Canada.
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| pubmed:publicationType |
Journal Article
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