Source:http://linkedlifedata.com/resource/pubmed/id/18601069
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2008-7-8
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| pubmed:abstractText |
Highly branched (colonial) mutants (MC1-1-, CC1-1, and C106) of Fusarium graminearum A3/5 were each grown with the parental strain (A3/5) in continuous flow cultures at high and low dilution rates using a variety of nutrient limitations. MC1-1 replaced A3/5 in all nutrient-limited cultures tested (glucose-, Mg(2+)-, ammonium-, and sulphate-limited cultures), suggesting that it has a higher maximum specific growh rate than A3/5. Compared with A3/5, C106 was positively selected for in Mg(2+)-limited cultures and its selection coefficient was higher at low than at high dilution rates, suggesting that, compared with A3/5, it has a reduced saturation constant (K(s)) for Mg(2+). However, in batch culture, C106 and A3/5 had the same (15 microM) appaent K(s) value for Mg(2+). C106 was replaced (negative selection coefficient) by A3/5 in gluose-, ammonium-, and phsophate-limited continuous flow cultures, but was neither at an advantage nor a disadvantage (i.e., it behaved as a neutral mutation) in sulphate-limited cultures. CC1-1 replaced A3/5 when they were grown together in glucose-, maltose-, or ribose-limited continuous flow cultures, but not in fructose-, xylose-, ammonium-, or phsophate-limited cultures. Because A3/5 and CC1-1 had similar K(m) values (30 microM) for glucose, and because the selective advantage of CC1-1 was maintained in maltose-limited cultures (maltose was not hydrolyzed extracellularly), it was concluded that the selective advantage of CC1-1 did not result from it having a lower K(s) for glucose than the parental strain. Rather, the data suggested that the activity of phosphoketopentoepimerase may be altered by the CC1-1 mutation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0006-3592
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| pubmed:author | |
| pubmed:copyrightInfo |
(c) 1992 John Wiley & Sons, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
5
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| pubmed:volume |
40
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1181-9
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| pubmed:year |
1992
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| pubmed:articleTitle |
Nutrient-dependent selection of morphological mutants of Fusarium graminearum A3/5 isolated from long-term continuous flow cultures.
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| pubmed:affiliation |
Microbiology Research Group, Department of Cell and Structural Biology, School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK.
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| pubmed:publicationType |
Journal Article
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