Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7205
pubmed:dateCreated
2008-8-7
pubmed:databankReference
pubmed:abstractText
DNA methylation is essential for normal development and has been implicated in many pathologies including cancer. Our knowledge about the genome-wide distribution of DNA methylation, how it changes during cellular differentiation and how it relates to histone methylation and other chromatin modifications in mammals remains limited. Here we report the generation and analysis of genome-scale DNA methylation profiles at nucleotide resolution in mammalian cells. Using high-throughput reduced representation bisulphite sequencing and single-molecule-based sequencing, we generated DNA methylation maps covering most CpG islands, and a representative sampling of conserved non-coding elements, transposons and other genomic features, for mouse embryonic stem cells, embryonic-stem-cell-derived and primary neural cells, and eight other primary tissues. Several key findings emerge from the data. First, DNA methylation patterns are better correlated with histone methylation patterns than with the underlying genome sequence context. Second, methylation of CpGs are dynamic epigenetic marks that undergo extensive changes during cellular differentiation, particularly in regulatory regions outside of core promoters. Third, analysis of embryonic-stem-cell-derived and primary cells reveals that 'weak' CpG islands associated with a specific set of developmentally regulated genes undergo aberrant hypermethylation during extended proliferation in vitro, in a pattern reminiscent of that reported in some primary tumours. More generally, the results establish reduced representation bisulphite sequencing as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-10688657, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-11005794, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-11029002, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-11440994, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-11782440, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-12923295, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-1542678, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-15489135, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-15680324, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-15809261, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-16086633, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-16224102, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-16432200, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-16627686, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-16630819, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-16870691, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17072317, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17085482, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17277777, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17283119, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17320506, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17334365, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17457052, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17467259, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17522676, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17603471, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-17687327, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-18232738, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-18278030, http://linkedlifedata.com/resource/pubmed/commentcorrection/18600261-1968664
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1476-4687
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
454
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
766-70
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Genome-scale DNA methylation maps of pluripotent and differentiated cells.
pubmed:affiliation
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural