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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5885
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pubmed:dateCreated |
2008-7-7
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pubmed:abstractText |
After fertilization, maternal proteins in oocytes are degraded and new proteins encoded by the zygotic genome are synthesized. We found that autophagy, a process for the degradation of cytoplasmic constituents in the lysosome, plays a critical role during this period. Autophagy was triggered by fertilization and up-regulated in early mouse embryos. Autophagy-defective oocytes derived from oocyte-specific Atg5 (autophagy-related 5) knockout mice failed to develop beyond the four- and eight-cell stages if they were fertilized by Atg5-null sperm, but could develop if they were fertilized by wild-type sperm. Protein synthesis rates were reduced in the autophagy-null embryos. Thus, autophagic degradation within early embryos is essential for preimplantation development in mammals.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-20
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pubmed:meshHeading |
pubmed-meshheading:18599786-Animals,
pubmed-meshheading:18599786-Autophagy,
pubmed-meshheading:18599786-Blastocyst,
pubmed-meshheading:18599786-Cells, Cultured,
pubmed-meshheading:18599786-Embryonic Development,
pubmed-meshheading:18599786-Female,
pubmed-meshheading:18599786-Fertilization,
pubmed-meshheading:18599786-Lysosomes,
pubmed-meshheading:18599786-Male,
pubmed-meshheading:18599786-Mice,
pubmed-meshheading:18599786-Mice, Knockout,
pubmed-meshheading:18599786-Mice, Transgenic,
pubmed-meshheading:18599786-Microtubule-Associated Proteins,
pubmed-meshheading:18599786-Oocytes,
pubmed-meshheading:18599786-Parthenogenesis,
pubmed-meshheading:18599786-Phagosomes,
pubmed-meshheading:18599786-Pregnancy,
pubmed-meshheading:18599786-Protein Biosynthesis,
pubmed-meshheading:18599786-Proteins,
pubmed-meshheading:18599786-Recombinant Fusion Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
Autophagy is essential for preimplantation development of mouse embryos.
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pubmed:affiliation |
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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