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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
30
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pubmed:dateCreated |
2008-7-25
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pubmed:abstractText |
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the chemically synthesized proteins d-sfAFP and l-sfAFP occurred much more readily than for l-sfAFP alone. More facile crystal formation also occurred from a quasi-racemic mixture of d-sfAFP and l-Se-sfAFP, a chemical protein analogue that contains an additional -SeCH2- moiety at one residue and thus differs slightly from the true enantiomer. Multiple wavelength anomalous dispersion (MAD) phasing from quasi-racemate crystals was then used to determine the X-ray structure of the sfAFP protein molecule. The resulting model was used to solve by molecular replacement the X-ray structure of l-sfAFP to a resolution of 0.98 A. The l-sfAFP molecule is made up of six antiparallel left-handed PPII helixes, stacked in two sets of three, to form a compact brick-like structure with one hydrophilic face and one hydrophobic face. This is a novel experimental protein structure and closely resembles a structural model proposed for sfAFP. These results illustrate the utility of total chemical synthesis combined with racemic crystallization and X-ray crystallography for determining the unknown structure of a protein.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-10089316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-10422829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-10917536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-10966479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-12015145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-12171656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-12351820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-15127445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-15299926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-1566069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-16239469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-16683747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-16704265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-17158562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-17360367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-18598026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-5488456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-5574522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-5764871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-7773780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-7973629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-8346193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-8846217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-9048383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-9261867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18598029-9878408
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1520-5126
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
30
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9695-701
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers.
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pubmed:affiliation |
Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois 60637, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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