Linked Life Data

18598009

Source:http://linkedlifedata.com/resource/pubmed/id/18598009

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
2008-7-8
pubmed:abstractText
The potential physiological relevance of liquid-liquid phase separation in lipid membranes to the formation and stability of "lipid rafts" in cellular plasma membranes has prompted extensive investigation of the physical chemistry underlying these phenomena. In this contribution, the line tension (gamma) and dipole density differences ( micro) between demixed fluid phases of monolayers comprised of dimyristoylphosphatidylcholine (DMPC) and dihydrocholesterol (DChol) were investigated by measuring the two-dimensional thermal fluctuations of domain boundaries visualized by the inclusion of a fluorescent tracer lipid. These parameters are essential determinants of domain stability, and their quantification will yield an increased understanding of the physical processes responsible for aspects of lateral phase separation. Employing an extensive data set, the surface pressure dependence of gamma and mu was determined at three different monolayer compositions (30%, 35%, and 40% DChol). Both parameters were found to decrease with a power law dependence as the surface pressure approached the phase transition pressure (pi t), in agreement with previous measurements. Additionally, photobleaching effects and domain size influence were quantified and found to be small in our system. We suggest that the method of flicker spectroscopy can be helpful in identifying line-active compounds.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1520-6106
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8063-8
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Critical exponents for line tension and dipole density difference from lipid monolayer domain boundary fluctuations.
pubmed:affiliation
Department of Chemistry, Institute for Medicine and Engineering, and Laboratory for Research on the Structure of Matter, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't