Linked Life Data

18597080

Source:http://linkedlifedata.com/resource/pubmed/id/18597080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-8-18
pubmed:abstractText
A one-step homogenous sensitive immunoassay using surface-enhanced Raman scattering (SERS) has been developed. This strategy is based on the aggregation of Raman reporter-labeled immunogold nanoparticles induced by the immunoreaction with corresponding antigens. The aggregation of gold nanoparticles results in a SERS signal increase of the Raman reporter. Therefore, human IgG could be directly determined by measuring the Raman signal of the reporter. The process of aggregation was investigated by transmission electron microscopy (TEM) and UV-Vis absorption spectroscopy. The effects of the temperature, time, and size of gold nanoparticles on the sensitivity of the assay were examined. Using human IgG as a model protein, a wide linear dynamic range (0.1-15 microg mL(-1)) was reached with low detection limit (0.1 microg mL(-1)) under optimized assay conditions. The successful test suggests that the application of the proposed method holds promising potential for simple, fast detection of proteins in the fields of molecular biology and clinical diagnostics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
392
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
187-93
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Immunoassay using surface-enhanced Raman scattering based on aggregation of reporter-labeled immunogold nanoparticles.
pubmed:affiliation
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't