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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2008-7-3
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pubmed:abstractText |
Odour-mediated signal transduction is a complex process that occurs in the cilia of olfactory sensory neurons. To gain insight in to the molecular organization of the odour transduction machinery, we developed a procedure to purify olfactory cilia membranes by differential centrifugation of rat olfactory epithelium extracts. We tested whether known scaffolding proteins that might participate in the assembly of the complex chemotransduction apparatus are present in the purified membrane fraction. Utilizing immunoblotting and immunohistochemistry, we show that the multidomain scaffolding proteins ProSAP/Shanks and calcium/calmodulin-dependent serine protein kinase CASK are present in the olfactory cilia. Ion channels involved in chemotransduction could be reconstituted into planar lipid bilayers for electrophysiological recordings. Our procedure should allow the identification of further chemotransduction-related proteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/CASK kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cnga2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic Nucleotide-Gated Cation...,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Olfactory Marker Protein,
http://linkedlifedata.com/resource/pubmed/chemical/ProSAPiP1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Shank3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/adenylate cyclase 3
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0959-4965
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pubmed:author |
pubmed-author:BacigalupoJuanJ,
pubmed-author:CastilloKarenK,
pubmed-author:DelgadoMaría GracielaMG,
pubmed-author:DelgadoRicardoR,
pubmed-author:GundelfingerEckart DED,
pubmed-author:OlavarriaKarelK,
pubmed-author:SaavedraMaría VerónicaMV,
pubmed-author:SandovalSoledadS,
pubmed-author:SmallaKarl-HeinzKH,
pubmed-author:ThomasUlrichU,
pubmed-author:WynekenUrsulaU
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1123-6
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pubmed:dateRevised |
2009-9-4
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pubmed:meshHeading |
pubmed-meshheading:18596612-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:18596612-Adenylate Cyclase,
pubmed-meshheading:18596612-Animals,
pubmed-meshheading:18596612-Blotting, Western,
pubmed-meshheading:18596612-Carrier Proteins,
pubmed-meshheading:18596612-Cilia,
pubmed-meshheading:18596612-Cyclic Nucleotide-Gated Cation Channels,
pubmed-meshheading:18596612-Guanylate Kinase,
pubmed-meshheading:18596612-Immunohistochemistry,
pubmed-meshheading:18596612-Ion Channels,
pubmed-meshheading:18596612-Isoenzymes,
pubmed-meshheading:18596612-Lipid Bilayers,
pubmed-meshheading:18596612-Membrane Proteins,
pubmed-meshheading:18596612-Olfactory Marker Protein,
pubmed-meshheading:18596612-Olfactory Mucosa,
pubmed-meshheading:18596612-Olfactory Receptor Neurons,
pubmed-meshheading:18596612-Patch-Clamp Techniques,
pubmed-meshheading:18596612-Rats,
pubmed-meshheading:18596612-Rats, Sprague-Dawley,
pubmed-meshheading:18596612-Signal Transduction
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pubmed:year |
2008
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pubmed:articleTitle |
Scaffolding proteins in highly purified rat olfactory cilia membranes.
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pubmed:affiliation |
Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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