| pubmed-article:1858912 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1858912 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:1858912 | lifeskim:mentions | umls-concept:C0003847 | lld:lifeskim |
| pubmed-article:1858912 | lifeskim:mentions | umls-concept:C0700124 | lld:lifeskim |
| pubmed-article:1858912 | lifeskim:mentions | umls-concept:C1425284 | lld:lifeskim |
| pubmed-article:1858912 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:1858912 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:1858912 | pubmed:issue | 1 Pt 2 | lld:pubmed |
| pubmed-article:1858912 | pubmed:dateCreated | 1991-8-23 | lld:pubmed |
| pubmed-article:1858912 | pubmed:abstractText | Microvascular endothelial cells (MECs) from rat epididymal fat pad were isolated and cultured in vitro on Cytodex 3 microcarrier beads. In Krebs-suffused cremaster muscle of pentobarbital-anesthetized rats arteriolar diameters (mean control diam 20.9 +/- 0.9 micron) were measured using image shearing video microscopy. Two lines of suffusate (1.5 ml/min each) were established; one contained a column of microcarrier beads only (no cells in line; NC) the other contained a 1-ml column of MECs grown on beads (through cells; TC). The muscle preparation and the MECs were first treated with indomethacin (Indo; 28 microM). Indo treatment blocked arteriolar dilation to A23187 (1 microM) and arachidonic acid (AA; 0.25 microM) administered into the NC line. A 4.0 +/- 0.6 micron increase in arteriolar diameter was observed, however, when A23187 (but not AA) was infused through the TC line containing Indotreated MECs on beads. The A23187-elicited dilation was abolished by the introduction of NG-monomethyl-L-arginine (L-NMMA; 200 microM) into the TC line. Administration of atropine (2 microM) onto the cremaster muscle via the NC line inhibited the dilations in response to acetylcholine (ACh; 2.7 microM) given through the NC line. Infusion of ACh through the TC line onto the atropine-treated cremaster muscle, however, elicited a 5.8 +/- 1.3 micron increase in arteriolar diameter, a response that was blocked by prior administration of L-NMMA into the TC line. Arteriolar dilation induced by adenosine (0.5 microM) or sodium nitroprusside (0.5 microM) applied via the NC or TC line was unaffected by L-NMMA.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:1858912 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1858912 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1858912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1858912 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1858912 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1858912 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:1858912 | pubmed:author | pubmed-author:KollerAA | lld:pubmed |
| pubmed-article:1858912 | pubmed:author | pubmed-author:GerritsenM... | lld:pubmed |
| pubmed-article:1858912 | pubmed:author | pubmed-author:KaleyGG | lld:pubmed |
| pubmed-article:1858912 | pubmed:author | pubmed-author:SeyediNN | lld:pubmed |
| pubmed-article:1858912 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1858912 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:1858912 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1858912 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1858912 | pubmed:pagination | H128-33 | lld:pubmed |
| pubmed-article:1858912 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:meshHeading | pubmed-meshheading:1858912-... | lld:pubmed |
| pubmed-article:1858912 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1858912 | pubmed:articleTitle | EDRF released from microvascular endothelial cells dilates arterioles in vivo. | lld:pubmed |
| pubmed-article:1858912 | pubmed:affiliation | Department of Physiology, New York Medical College, Valhalla 10595. | lld:pubmed |
| pubmed-article:1858912 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1858912 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1858912 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
