|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2008-8-29
|
|
pubmed:abstractText |
The interferon (IFN) antagonists of Japanese encephalitis virus (JEV) proteins contribute to the JE pathogenesis. Most flavivirus non-structural (NS) proteins correlate with virus-induced inflammation and immune escape. NS4A proteins of West Nile virus and dengue type 2 virus have been demonstrated to inhibit IFN signaling. In this study, JEV NS4A without the C-terminal 2K domain has been demonstrated to partially block activation of an IFN-stimulated response element (ISRE)-based cis-reporter by IFN-alpha/beta. In addition, JEV NS4A significantly inhibited the phosphorylation levels of STAT1 and STAT2, but not TYK2 in the IFN-treated cells. Moreover, the N-terminus of a RNA helicase DDX42 protein identified using a phage display human brain cDNA library have been demonstrated to specifically bind to JEV NS4A in vitro using a co-immunoprecipitation assay. The interaction between JEV NS4A and RNA helicase DDX42 showed partial co-localization in human medulloblastoma TE-671 cells by confocal microscopy. Importantly, the expression of N-terminal DDX42 is able to overcome JEV-induced antagonism of IFN responses. Therefore, these results show that JEV NS4A without the C-terminal 2K domain is associated with modulation of the IFN response and the interaction of JEV NS4A with RNA helicase DDX42 could be important for JE pathogenesis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DDX42 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoprotein, U2 Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/STAT Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/red fluorescent protein
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0168-1702
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
137
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
49-55
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:18588927-Animals,
pubmed-meshheading:18588927-Cell Line, Tumor,
pubmed-meshheading:18588927-Cercopithecus aethiops,
pubmed-meshheading:18588927-Cricetinae,
pubmed-meshheading:18588927-DEAD-box RNA Helicases,
pubmed-meshheading:18588927-Encephalitis, Japanese,
pubmed-meshheading:18588927-Encephalitis Virus, Japanese,
pubmed-meshheading:18588927-Gene Expression Regulation, Viral,
pubmed-meshheading:18588927-Gene Library,
pubmed-meshheading:18588927-Green Fluorescent Proteins,
pubmed-meshheading:18588927-Humans,
pubmed-meshheading:18588927-Interferon Type I,
pubmed-meshheading:18588927-Janus Kinases,
pubmed-meshheading:18588927-Luminescent Proteins,
pubmed-meshheading:18588927-Microscopy, Confocal,
pubmed-meshheading:18588927-Phosphorylation,
pubmed-meshheading:18588927-Protein Binding,
pubmed-meshheading:18588927-Recombinant Fusion Proteins,
pubmed-meshheading:18588927-Ribonucleoprotein, U2 Small Nuclear,
pubmed-meshheading:18588927-STAT Transcription Factors,
pubmed-meshheading:18588927-Signal Transduction,
pubmed-meshheading:18588927-Vero Cells,
pubmed-meshheading:18588927-Viral Proteins
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Interferon antagonist function of Japanese encephalitis virus NS4A and its interaction with DEAD-box RNA helicase DDX42.
|
|
pubmed:affiliation |
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan. cwlin@mail.cmu.edu.tw
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
