Linked Life Data

18588908

Source:http://linkedlifedata.com/resource/pubmed/id/18588908

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-8-1
pubmed:abstractText
In vertebrates, non-lens betagamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive. betagamma-CAT is the first example of a naturally existing multifunctional protein complex of a non-lens betagamma-crystallin and a trefoil factor from frog Bombina maxima skin secretions. Here we report the investigation of its in vivo toxic effects on mice and rabbits. The LD(50) values of betagamma-CAT on mice were determined to be 0.4 mg/kg and 20 microg/kg under intraperitoneal (i.p.) and intravenous (i.v.) injection, respectively. The mice subcutaneously injected with betagamma-CAT (6 microg/g body weight) showed strong hyperaemia of subcutaneous capillary vessel, but no hemorrhagic spots were observed. Intravenous injection of betagamma-CAT in rabbits (8-22 microg/kg body weight) caused a rapidly hypotensive effect and followed with cardiovascular collapse. Injection with betagamma-CAT (22 microg/kg, i.v.) significantly decreased hematocrit (P<0.05) and mean corpuscular volume (P<0.05) of the rabbits in 5 min. At the same time, the counts of platelets and white blood cells were significantly decreased (P<0.05), while the blood levels of glucose, lactate dehydrogenase and serum glutamic-oxaloacetic transaminase were significantly increased (P<0.05). Furthermore, serials of tissues edema and damages were also observed. These results indicate that betagamma-CAT rapidly caused several in vivo toxic effects on mammals and its lethal toxic potency was mainly contributed by hypotension and cardiovascular collapse, providing new clues for the understanding of the patho-physiological roles of non-lens betagamma-crystallins and trefoil factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22-31
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Acute toxicity of betagamma-CAT, a naturally existing non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions.
pubmed:affiliation
Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't