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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2008-11-12
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pubmed:abstractText |
Human herpesvirus-8 (HHV-8) is the causative agent of Kaposi's sarcoma and is associated with the angioproliferative disorders primary effusion lymphoma and multicentric Castleman's disease. Evidence of HHV-8 infection within the pulmonary vasculature of patients with idiopathic pulmonary arterial hypertension (IPAH) has been described. We hypothesize that HHV-8 infection of pulmonary microvascular endothelial cells results in an apoptotic-resistant phenotype characteristic of severe pulmonary arterial hypertension. Our objective was to investigate the ability of HHV-8 to infect human pulmonary microvascular endothelial cells in vitro and characterize the phenotypic effect of this infection. Human pulmonary microvascular endothelial cells were exposed to HHV-8 using two methods (direct virus and co-culture technique). The presence of lytic and latent infection was confirmed. Changes in endothelial cell gene and protein expression and effects on cellular apoptosis were measured. HHV-8 can both lytically and latently infect primary human pulmonary microvascular endothelial cells in vitro. HHV-8 infection results in significant changes in gene expression, including alterations of pathways important to cellular apoptosis. HHV-8 infection also alters expression of genes integral to the bone morphogenic protein pathway, including down-regulation of bone morphogenic protein-4. Other genes previously implicated in the development of PAH are affected by HHV-8 infection, and cells infected with HHV-8 are resistant to apoptosis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-10361101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-10767252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-10903931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-10969041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-10973254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11015450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11094047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11282888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11462044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11502704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11686884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11757635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11762997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-11884566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12004244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12134042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12384956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12415595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12538164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12571257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12778132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-12828857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-13679525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-14516126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-14729610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15194174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15194176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15194184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15215156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15220918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15578080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15764755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15845886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-15863640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-16192453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-16200212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-16229789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-16264059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-16678088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-16884948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-17075601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-17102831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-17188706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-17483315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-1954381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-7508683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-7580295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-7632932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-7700311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-7735624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-7997879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-8612236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-8896516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18587055-9741573
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1535-4989
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
706-16
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pubmed:dateRevised |
2010-9-21
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pubmed:meshHeading |
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