Source:http://linkedlifedata.com/resource/pubmed/id/18586005
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2008-7-28
|
| pubmed:abstractText |
Transforming growth factor-beta1 (TGF-beta1) is a multi-functional cytokine involved in the regulation of cell proliferation, differentiation and extracellular matrix formation. In search for novel genes mediating the TGF-beta1 function at downstream signaling, we performed a cDNA microarray analysis and identified 60 genes whose expression is regulated by TGF-beta1 in the liver cancer cell line PLC/PRF/5. Among them, we report here lysyl oxidase like 4 (LOXL4) as a novel target of TGF-beta1 signaling, and provide experimental evidence for its expression regulation and function. LOXL4 was found to be the only member of LOX family whose expression is induced by TGF-beta1 in hepatoma cells. Deletion mapping of the LOXL4 promoter indicated that the TGF-beta1 regulation of LOXL4 expression is mediated through the binding of AP1 transcription factor to a conserved region of the promoter. This was confirmed by the chromatin immunoprecipitation assay that captured c-Fos-bound chromatin from TGF-beta1-treated cells. Forced expression of LOXL4 in PLC/PRF/5 cells resulted in inhibition of cell motility through Matrigel in the presence of TGF-beta1 treatment. In parallel, LOXL4 suppressed the expression of laminins and alpha3 integrin and the activity of MMP2. These results suggest that LOXL4 may function as a negative feedback regulator of TGF-beta1 in cell invasion by inhibiting the metabolism of extracellular matrix (ECM) components.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/LOXL4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
1090-2104
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| pubmed:author |
pubmed-author:BaeEun MiEM,
pubmed-author:KangDong ChulDC,
pubmed-author:KimDong JoonDJ,
pubmed-author:KimDong MinDM,
pubmed-author:KimSoo JungSJ,
pubmed-author:LeeDong ChulDC,
pubmed-author:LeeJung JuJJ,
pubmed-author:MinSang HyunSH,
pubmed-author:MyungPyung KeunPK,
pubmed-author:ParkKyung ChanKC,
pubmed-author:SangByung ChanBC,
pubmed-author:YangSuk-JinSJ,
pubmed-author:YeomYoung IlYI
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
5
|
| pubmed:volume |
373
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
521-7
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:18586005-Amino Acid Oxidoreductases,
pubmed-meshheading:18586005-Carcinoma, Hepatocellular,
pubmed-meshheading:18586005-Cell Line, Tumor,
pubmed-meshheading:18586005-Cell Movement,
pubmed-meshheading:18586005-Extracellular Matrix,
pubmed-meshheading:18586005-Feedback, Physiological,
pubmed-meshheading:18586005-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:18586005-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18586005-Humans,
pubmed-meshheading:18586005-Liver Neoplasms,
pubmed-meshheading:18586005-Neoplasm Invasiveness,
pubmed-meshheading:18586005-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18586005-Transcription, Genetic,
pubmed-meshheading:18586005-Transcription Factor AP-1,
pubmed-meshheading:18586005-Transforming Growth Factor beta1
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Lysyl oxidase like 4, a novel target gene of TGF-beta1 signaling, can negatively regulate TGF-beta1-induced cell motility in PLC/PRF/5 hepatoma cells.
|
| pubmed:affiliation |
Medical Genomics Research Center, KRIBB, P.O. Box 115, Yusong, Daejeon 305-806, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|