Linked Life Data

18585149

Source:http://linkedlifedata.com/resource/pubmed/id/18585149

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-30
pubmed:abstractText
A novel immunoassay based on surface-enhanced Raman scattering (SERS) has been developed. The method exploits the SERS-derived signal from reporter molecules (crystal violet, CV) encapsulated in antibody-modified liposome particles. The antigen is firstly captured by the primary antibody immobilized in microwell plates and then sandwiched by secondary antibody-modified liposome. The CV molecules are released from the liposome and transferred to specially designed substrate of gold nanosphere arrays with sub-10-nm gaps. The concentration of the antigen is indirectly read out by the SERS intensity of the CVs. The substrate used could substantially improve the sensitivity and reproducibility of SERS measurement. The SERS intensity responses are linearly correlated to logarithm of antigen concentration in the range of 1.0 x 10(-8) to 1.0 x 10(-4) gm L(-1) with a detection limit of 8 ng mL(-1). To our knowledge, this is the first report describing liposome-mediated enhancement of the sensitivity in immunoassay based on surface-enhanced Raman scattering. Experimental results show that the proposed method illustrates a potential prospect of applications in immunoassay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-3573
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
797-803
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Liposome-mediated enhancement of the sensitivity in immunoassay based on surface-enhanced Raman scattering at gold nanosphere array substrate.
pubmed:affiliation
State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha, Hunan Province 410082, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't