Linked Life Data

18585129

Source:http://linkedlifedata.com/resource/pubmed/id/18585129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-30
pubmed:abstractText
In this paper, electrochemical surface plasmon resonance (SPR) method was first used to detect enzymatic reaction in bilayer lipid membrane (BLM) based on immobilizing horseradish peroxidase (HRP) in the BLMs supported by the redox polyaniline (PAn) film. By SPR kinetic curve in situ monitoring the redox transformation of PAn film resulted from the reaction between HRP and PAn, the enzymatic reaction of HRP with H(2)O(2) was successfully analyzed by electrochemical SPR spectroscopy. The results show that this BLM supported on PAn film cannot only preserve the bioactivity of HRP immobilized in the membrane, but also provide a channel for the transfer of electrons between HRP and PAn on electrode surface. These characteristics enabled the development of SPR biosensor for sensitively detecting H(2)O(2). H(2)O(2) has been detected by electrochemical SPR spectroscopy in the concentration range of 5 x 10(-5)M to 2 x 10(-3)M. After each of detections, the SPR sensor surface was completely regenerated by electrochemically reducing the oxidized PAn to its reduced state. This method provides a novel route for enhancing the detection of small ligand of enzymatic reaction in BLM by electrochemical SPR spectroscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-3573
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
666-70
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Electrochemical surface plasmon resonance detection of enzymatic reaction in bilayer lipid membranes.
pubmed:affiliation
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun Jilin 130022, Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't