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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-9-8
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pubmed:abstractText |
5-FU is commonly used for treatment of various solid tumors including colon carcinoma. We have previously demonstrated that Egr-1 induced by 5-FU enhanced TSP-1 expression in human colon cancer KM12C cells. In this study, a Genechip analysis of KM12C cells treated with 5-FU revealed down-regulation of 924 genes and up-regulation of 460 genes. The decreased expression of c-Myc mRNA and phosphorylated c-Myc were detected and confirmed by RT-PCR and immunoblotting. Since 5-FU induced the expression of TSP-1, we examined the effect of c-Myc on the TSP-1 promoter. Deletion of the TSP-1 promoter region in which binding sites for c-Myc reside had no effect on the TSP-1 promoter activity induced by 5-FU. Meanwhile, 5-FU dose-dependently decreased the expression of miR-17-92 cluster. These findings suggest that 5-FU decreased the expression of c-Myc and consequently miR-17-92 cluster and increased the expression of TSP-1 mRNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1872-7980
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pubmed:author |
pubmed-author:AkiyamaShin-ichiS,
pubmed-author:CheXiao-FangXF,
pubmed-author:FukushimaMasakazuM,
pubmed-author:FurukawaTatsuhikoT,
pubmed-author:HaraguchiMisakoM,
pubmed-author:IkedaRyujiR,
pubmed-author:IwashitaKen-ichiK,
pubmed-author:KuwanoMichihikoM,
pubmed-author:NakagawaMasayukiM,
pubmed-author:OkaToshinoriT,
pubmed-author:OnoMayumiM,
pubmed-author:OoyamaAkioA,
pubmed-author:TabataShoS,
pubmed-author:YamamotoMasatatsuM,
pubmed-author:ZhaoHong-YeHY
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pubmed:issnType |
Electronic
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pubmed:day |
18
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
156-63
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:18583030-Angiogenesis Inhibitors,
pubmed-meshheading:18583030-Antimetabolites, Antineoplastic,
pubmed-meshheading:18583030-Cell Line, Tumor,
pubmed-meshheading:18583030-Colonic Neoplasms,
pubmed-meshheading:18583030-Down-Regulation,
pubmed-meshheading:18583030-Fluorouracil,
pubmed-meshheading:18583030-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18583030-Genes, myc,
pubmed-meshheading:18583030-Humans,
pubmed-meshheading:18583030-MicroRNAs,
pubmed-meshheading:18583030-Multigene Family,
pubmed-meshheading:18583030-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18583030-Promoter Regions, Genetic,
pubmed-meshheading:18583030-Thrombospondin 1
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pubmed:year |
2008
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pubmed:articleTitle |
Down regulation of c-Myc and induction of an angiogenesis inhibitor, thrombospondin-1, by 5-FU in human colon cancer KM12C cells.
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pubmed:affiliation |
Department of Molecular Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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