Linked Life Data

18581325

Source:http://linkedlifedata.com/resource/pubmed/id/18581325

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-7-1
pubmed:abstractText
Protracted inflammation has been associated with the generation of autoimmune responses. In this respect, increase in the chaperonin, heat shock protein 70 (hsp70) is an outcome of prolonged inflammatory stress. Previous experiments have shown that overexpression of inducible hsp70 in vitro enhanced myelin autoantigen recognition. To prove the role of hsp70 in myelin-directed responses in vivo, we applied a mouse deficient in the major gene encoding inducible hsp70, hsp70.1. Hsp70.1(-/-) mice sensitized for experimental autoimmune encephalomyelitis (EAE) with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55, displayed almost complete resistance to the disease. This correlated with the loss of T cell proliferation and IFN-gamma production in response to MOG(35-55). T cell transfer experiments as well as antigen presentation assays in vitro demonstrated that hsp70 deficiency was associated with dysfunction in the activation of autoreactive T cells. Moreover, T cell responses to ovalbumin (OVA) peptide 323-339 were altered and CD4(+) T cells were more prone to TCR-induced apoptosis, suggesting broader spectrum of T cell defect in hsp70.1(-/-) mice. These results provide compelling evidence for generalized effect mediated by inducible hsp70 in the recognition of myelin self and non-self antigens that influences the cytokine profile of the immune response affecting autoimmune demyelination.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1999-2013
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A heat shock protein gene (Hsp70.1) is critically involved in the generation of the immune response to myelin antigen.
pubmed:affiliation
Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, 22 Kopcinskiego Street, Lodz, Poland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural