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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1991-8-26
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pubmed:databankReference |
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pubmed:abstractText |
The beta antigen of the lbc protein complex of Group B streptococci is a cell-surface receptor which binds the Fc region of human immunoglobulin A (IgA). Determination of the nucleotide sequence of the beta antigen gene shows that it encodes a preprotein having a molecular weight of 130,963 daltons and a polypeptide of 1164 amino acid residues that is typical of other Gram-positive cell-wall proteins. There is a long signal sequence of 37 amino acids at the N-terminus. Four of the five C-terminal amino acid residues are basic and are preceded by a hydrophobic stretch that appears to anchor the C-terminus in the cell membrane. To the N-terminal side of this hydrophobic stretch is a putative cell-wall-spanning region containing proline-rich repeated sequences. An unusual feature of these repeated sequences is a three-residue periodicity, whereby every first residue is a proline, the second residue is alternating positively or negatively charged, and the third residue is uncharged. The IgA-binding activity was approximately localized by expressing subfragments of the beta antigen as fusion proteins. Two distinct but adjacent DNA segments specified peptides that bound IgA, which indicates that the IgA-binding activity is located in two distinct regions of the protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
843-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1857207-Amino Acid Sequence,
pubmed-meshheading:1857207-Antigens, Bacterial,
pubmed-meshheading:1857207-Bacterial Proteins,
pubmed-meshheading:1857207-Base Sequence,
pubmed-meshheading:1857207-Binding Sites,
pubmed-meshheading:1857207-Blotting, Western,
pubmed-meshheading:1857207-Chromosome Deletion,
pubmed-meshheading:1857207-Cloning, Molecular,
pubmed-meshheading:1857207-Immunoglobulin A,
pubmed-meshheading:1857207-Molecular Sequence Data,
pubmed-meshheading:1857207-Receptors, Fc,
pubmed-meshheading:1857207-Recombinant Fusion Proteins,
pubmed-meshheading:1857207-Streptococcus agalactiae
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pubmed:year |
1991
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pubmed:articleTitle |
The IgA-binding beta antigen of the c protein complex of Group B streptococci: sequence determination of its gene and detection of two binding regions.
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pubmed:affiliation |
Department of Microbiology, Technical University, Braunschweig, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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