Source:http://linkedlifedata.com/resource/pubmed/id/18569387
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2008-6-23
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| pubmed:abstractText |
Stem cell factor (SCF) is not only critical for mast cell development, but also an important mast cell functional regulator. However, roles of transcription factors involved in SCF-induced effects remain incompletely defined. Early growth response factor-1 (Egr-1) is a member of zinc-finger transcription factor family. Mouse bone marrow-derived mast cells (BMMC) were used to examine a role of Egr-1 in SCF-induced mast cell activation and growth. SCF induced a strong and rapid expression of Egr-1 mRNA as tested by real-time PCR analysis. SCF-induced Egr-1 nuclear translocation and DNA binding were demonstrated by electrophoretic mobility shift assay (EMSA) and immunofluorescence assay. To examine if Egr-1 is required for SCF-induced IL-13 expression, Egr-1-deficient BMMC were used. Levels of SCF-induced IL-13 mRNA and protein were reduced in Egr-1 deficient BMMC when compared with wild-type BMMC. Although Egr-1 is required for macrophage and lymphocyte development, SCF-induced mast cells growth was not affected by Egr-1 deficiency. Interestingly, SCF-induced Egr activation was blocked by a tyrosine kinase inhibitor PP2, suggesting a role of tyrosine phosphorylation in SCF-induced Egr-1 activation. Taken together, our results suggest that Egr-1 is required for SCF-induced IL-13 expression, but not mast cell growth.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AG 1879,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Egr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1547-6901
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
5
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
163-71
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:18569387-Active Transport, Cell Nucleus,
pubmed-meshheading:18569387-Animals,
pubmed-meshheading:18569387-Bone Marrow Cells,
pubmed-meshheading:18569387-Cell Nucleus,
pubmed-meshheading:18569387-Cells, Cultured,
pubmed-meshheading:18569387-Early Growth Response Protein 1,
pubmed-meshheading:18569387-Humans,
pubmed-meshheading:18569387-Interleukin-13,
pubmed-meshheading:18569387-Lymphocytes,
pubmed-meshheading:18569387-Macrophages,
pubmed-meshheading:18569387-Mast Cells,
pubmed-meshheading:18569387-Mice,
pubmed-meshheading:18569387-Mice, Knockout,
pubmed-meshheading:18569387-Protein Kinase Inhibitors,
pubmed-meshheading:18569387-Protein-Tyrosine Kinases,
pubmed-meshheading:18569387-Pyrimidines,
pubmed-meshheading:18569387-RNA, Messenger,
pubmed-meshheading:18569387-Stem Cell Factor
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| pubmed:year |
2008
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| pubmed:articleTitle |
The early growth response factor-1 contributes to interleukin-13 production by mast cells in response to stem cell factor stimulation.
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| pubmed:affiliation |
Department of Microbiology and Immunology, Izaak Walton Killam Health Center, Dalhousie University, Halifax, Nova Scotia, Canada.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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