Linked Life Data

18569374

Source:http://linkedlifedata.com/resource/pubmed/id/18569374

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-23
pubmed:abstractText
A novel chemiluminescence immunoassay (CLIA) of total thyroxin (TT4) with the acridinium ester of DMAE x NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] labeled has been developed. In our method, microwells were coated with anti-T4 monoclonal antibody (McAb) and DMAE x NHS synthesized by our laboratory was conjugated with streptavidin (SA). T4-BSA was conjugated with biotin-N-hydroxysuccinimide (B x NHS), T4-BSA- B x NHS and the T4 in the standard or sample competitively react with anti-T4 McAb. Streptavidin-biotin separation and enhanced techniques were applied in this assay. The presented approach shows many excellent characteristics, particularly, rapid assay process and high detection sensitivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1532-4230
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-65
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE x NHS-Labeled.
pubmed:affiliation
Shanghai Applied Radiation Institute, College of Environmental and Chemical Engineering, Shanghai University, Jiadin, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't