Linked Life Data

18567993

Source:http://linkedlifedata.com/resource/pubmed/id/18567993

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2008-8-26
pubmed:abstractText
The MYC onco-protein is a transcription factor that regulates cell proliferation, metabolism, protein synthesis, mitochondrial function and stem cell renewal. A region on chromosome 8q24 encompassing the MYC locus is amplified in prostate cancer, but this occurs mostly in advanced disease suggesting that MYC alterations occur late in prostate cancer. In contrast, MYC mRNA is elevated in most prostate cancers, even those of relatively low stage and grade (eg Gleason score 6) suggesting that MYC plays a role in initiation. However, since MYC protein levels are tightly regulated, elevated MYC mRNA does not necessarily imply elevated MYC protein. Thus, it is critical to determine whether MYC protein is elevated in human prostate cancer, and if so, at what stage of the disease this elevation occurs. Prior studies of MYC protein localization have been hampered by lack of suitable antibodies and controls. We utilized a new anti-MYC antibody coupled with genetically defined control experiments to localize MYC protein within human tissue microarrays consisting of normal, atrophy, PIN, primary adenocarcinoma, and metastatic adenocarcinoma. Nuclear overexpression of MYC protein occurred frequently in luminal cells of PIN, as well as in most primary carcinomas and metastatic disease. MYC protein did not correlate with gain of 8q24, suggesting alternative mechanisms for MYC overexpression. These results provide evidence that upregulation of nuclear MYC protein expression is a highly prevalent and early change in prostate cancer and suggest that increased nuclear MYC may be a critical oncogenic event driving human prostate cancer initiation and progression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1530-0285
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1156-67
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:18567993-Adenocarcinoma, pubmed-meshheading:18567993-Adult, pubmed-meshheading:18567993-Aged, pubmed-meshheading:18567993-Aged, 80 and over, pubmed-meshheading:18567993-Antibodies, Monoclonal, pubmed-meshheading:18567993-Cell Nucleus, pubmed-meshheading:18567993-Gene Expression Regulation, Neoplastic, pubmed-meshheading:18567993-Humans, pubmed-meshheading:18567993-Immunohistochemistry, pubmed-meshheading:18567993-Male, pubmed-meshheading:18567993-Middle Aged, pubmed-meshheading:18567993-Precancerous Conditions, pubmed-meshheading:18567993-Prostate, pubmed-meshheading:18567993-Prostatic Intraepithelial Neoplasia, pubmed-meshheading:18567993-Prostatic Neoplasms, pubmed-meshheading:18567993-Proto-Oncogene Proteins c-myc, pubmed-meshheading:18567993-RNA, Messenger, pubmed-meshheading:18567993-Tissue Array Analysis, pubmed-meshheading:18567993-Tumor Markers, Biological, pubmed-meshheading:18567993-Up-Regulation
pubmed:year
2008
pubmed:articleTitle
Nuclear MYC protein overexpression is an early alteration in human prostate carcinogenesis.
pubmed:affiliation
Department of Pathology, The Johns Hopkins University, School of Medicine, Baltimore, MD 21231, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural