rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-7-11
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pubmed:abstractText |
Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18565235-10560966,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2164
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pubmed:author |
|
pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
294
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pubmed:dateRevised |
2010-9-22
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pubmed:meshHeading |
pubmed-meshheading:18565235-Chromosomes, Plant,
pubmed-meshheading:18565235-DNA, Plant,
pubmed-meshheading:18565235-Flow Cytometry,
pubmed-meshheading:18565235-Genetic Markers,
pubmed-meshheading:18565235-Hordeum,
pubmed-meshheading:18565235-Nucleic Acid Amplification Techniques,
pubmed-meshheading:18565235-Physical Chromosome Mapping,
pubmed-meshheading:18565235-Polymerase Chain Reaction,
pubmed-meshheading:18565235-Polymorphism, Single Nucleotide
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pubmed:year |
2008
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pubmed:articleTitle |
Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley.
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pubmed:affiliation |
Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovská 6, CZ-77200 Olomouc, Czech Republic. simkovah@ueb.cas.cz
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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