Linked Life Data

18559559

Source:http://linkedlifedata.com/resource/pubmed/id/18559559

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-6-18
pubmed:abstractText
The measurement of the aflatoxin B(1)-lysine serum albumin adduct in human blood samples is the most facile biomarker for the assessment of chronic exposure to aflatoxin B(1). Many technologies have been developed for the measurement of this protein adduct including immunoassays, high-performance liquid chromatography (HPLC) with fluorescence detection, and a newly developed isotope-dilution mass spectrometry method. Irrespective of the technology used to determine this adduct level, an important question remains about the long-term stability of this damage product in stored samples. To address this issue, 19 human serum samples that had been previously analyzed for the aflatoxin B(1)-lysine adduct by high-performance liquid chromatography-fluorescence in 1989 were re-analyzed by isotope dilution mass spectrometry after storage at -80 degrees C. The adduct concentrations measured by these two techniques were identical within 4% over the range 5 to 100 pg of aflatoxin B(1)-lysine/mg albumin. In addition, the specific chemical structure of the aflatoxin B(1)-lysine adduct in human samples was confirmed for the first time by collision-induced dissociation full scan mass spectrometry analysis of the protonated adduct molecular ion. These results illustrate that the aflatoxin B(1)-lysine serum albumin adduct can be stable in human serum stored at -80 degrees C since 1989, and this provides confidence for the measurement of this biomarker in repository samples from epidemiologic investigations.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-12067245, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-1339083, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-16015671, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-16411655, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-16614131, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-17185282, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-17576701, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-2104776, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-2114222, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-2121383, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-266709, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-3085966, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-3111739, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-3133131, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-8448352, http://linkedlifedata.com/resource/pubmed/commentcorrection/18559559-8833618
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1055-9965
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1436-9
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Long-term stability of human aflatoxin B1 albumin adducts assessed by isotope dilution mass spectrometry and high-performance liquid chromatography-fluorescence.
pubmed:affiliation
Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205-2103, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural