Source:http://linkedlifedata.com/resource/pubmed/id/18555888
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2008-6-16
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pubmed:abstractText |
HIV-1 impairs the production of T cells, through mechanisms that are still unknown. Here, we investigated the effect of the expression of HIV-1 Nef on the T-cell potential of human hematopoietic CD34(+) precursors. Those progenitors were transduced by using lentiviral vectors expressing Nef and cultured on OP9-DL1 cells allowing the differentiation of T cell from human hematopoietic precursors. We demonstrate that Nef impairs the generation of a CD3epsilon(+)CD5(+) CD1a(+) precursor stage that has initiated a D-J rearrangement of the TCRbeta locus. Onward stages of T-cell development were also affected with a quantitative reduction of CD4(+) intraCD3epsilon(+) Immature single positive cells (ISP), Double Positive (DP) CD4(+)CD8(+) TCRalphabeta T cells and CD56(+) NK cells. But B cell production was not affected. Limiting dilution analyses demonstrated a significant reduction in the frequency of T/NK progenitors among Nef-expressing CD34(+) cells. Altogether, these data demonstrate that Nef interferes with the differentiation of a primitive lymphoid human precursor with a T/NK potential.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0042-6822
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pubmed:author |
pubmed-author:André-SchmutzIsabelleI,
pubmed-author:BrizziFannyF,
pubmed-author:Cavazzana-CalvoMarinaM,
pubmed-author:CoulombelLaureL,
pubmed-author:DorivalCélineC,
pubmed-author:EstaquierJérômeJ,
pubmed-author:HenryAdelineA,
pubmed-author:LévyYvesY,
pubmed-author:LelièvreJean-DanielJD,
pubmed-author:SchwartzOlivierO,
pubmed-author:SixEmmanuelleE,
pubmed-author:Sol-FoulonNathalieN
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
377
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-15
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pubmed:meshHeading |
pubmed-meshheading:18555888-Antigens, CD34,
pubmed-meshheading:18555888-Cell Differentiation,
pubmed-meshheading:18555888-Genes, nef,
pubmed-meshheading:18555888-HIV-1,
pubmed-meshheading:18555888-Hematopoietic Stem Cells,
pubmed-meshheading:18555888-Humans,
pubmed-meshheading:18555888-Killer Cells, Natural,
pubmed-meshheading:18555888-T-Lymphocyte Subsets,
pubmed-meshheading:18555888-Transduction, Genetic,
pubmed-meshheading:18555888-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2008
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pubmed:articleTitle |
HIV-1 Nef protein expression in human CD34+ progenitors impairs the differentiation of an early T/NK cell precursor.
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pubmed:affiliation |
INSERM U 841, Faculté de Médecine, Créteil, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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