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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-6-16
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pubmed:abstractText |
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/DA14546,
http://linkedlifedata.com/resource/pubmed/grant/GM63904,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-06A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-07S1,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA014546-08,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-03S1,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-06,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM063904-07,
http://linkedlifedata.com/resource/pubmed/grant/R56 GM063904-08
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1557-8542
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
121-3
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pubmed:dateRevised |
2011-3-23
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Zinc finger-based knockout punches for zebrafish genes.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA. Ekker.Stephen@mayo.edu
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pubmed:publicationType |
Journal Article,
Review,
Research Support, N.I.H., Extramural
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