Linked Life Data

18551418

Source:http://linkedlifedata.com/resource/pubmed/id/18551418

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-6-13
pubmed:abstractText
The synthesis of ribosomes is a major metabolic activity critical for cell growth and homeostasis. Understanding the mechanisms of ribosome biogenesis has important implications for studying both protein synthesis and cell cycle control. This unit describes several techniques for the analysis of rRNA maturation and ribosome assembly adapted for mammalian cells. Metabolic labeling of rRNA and hybridization analysis of precursors can be used to assess changes in rRNA processing that occur under experimental conditions of interest. Separation of preribosomal particles by sucrose gradient centrifugation is suitable for the analysis of proteins associated with preribosomes during their assembly and maturation in the cell nucleus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1934-2616
pubmed:author
pubmed:copyrightInfo
Copyright 2008 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 22.11
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Assays for ribosomal RNA processing and ribosome assembly.
pubmed:affiliation
University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Department of Cell Biology, Stratford, New Jersey, USA.
pubmed:publicationType
Journal Article