Linked Life Data

18550821

Source:http://linkedlifedata.com/resource/pubmed/id/18550821

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2008-6-19
pubmed:abstractText
Exocytosis of neurotransmitters and hormones occurs through the fusion of secretory vesicles with the plasma membrane. This highly regulated process involves key proteins, such as SNAREs, and specific lipids at the site of membrane fusion. Phospholipase D (PLD) has recently emerged as a promoter of membrane fusion in various exocytotic events potentially by providing fusogenic cone-shaped phosphatidic acid. We show here that PLD1 is regulated by ribosomal S6 kinase 2 (RSK2)-dependent phosphorylation. RSK2 is activated by a high K(+)-induced rise in cytosolic calcium. Expression of inactive RSK2 mutants or selective knockdown of endogenous RSK2 dramatically affects the different kinetic components of the exocytotic response in chromaffin cells. RSK2 physically interacts with and stimulates PLD activity through the phosphorylation of Thr-147 in the PLD1 amino-terminal phox homology domain. Expression of PLD1 phosphomimetic mutants fully restores secretion in cells depleted of RSK2, suggesting that RSK2 is a critical upstream signaling element in the activation of PLD1 to produce the lipids required for exocytosis. We propose that PLD-related defects in neuronal and endocrine activities could contribute to the effect observed after the loss-of-function mutations in Rsk2 that lead to Coffin-Lowry syndrome, an X-linked form of growth and mental retardation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-10195143, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-10411321, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-10433271, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-10980595, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-11113183, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-11350931, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-11752468, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-12119277, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-12362025, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-12379803, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-12414697, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-12876278, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-14527322, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-15052340, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-15087463, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-15182672, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-15635098, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-15980073, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16081518, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16143829, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16217014, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16439353, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16497229, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16638749, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-16912714, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-17001002, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-17028579, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-17502602, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-17540765, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-2002057, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-2842685, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-7498520, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-7563032, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-7686903, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-8278362, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-8700863, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-8955270, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-9430671, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-9446556, http://linkedlifedata.com/resource/pubmed/commentcorrection/18550821-9753324
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
17
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8434-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The Coffin-Lowry syndrome-associated protein RSK2 is implicated in calcium-regulated exocytosis through the regulation of PLD1.
pubmed:affiliation
Département Neurotransmission et Sécrétion Neuroendocrine, Institut des Neurosciences Cellulaires et Intégratives, Unité Mixte de Recherche 7168/LC2, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't