Linked Life Data

18549623

Source:http://linkedlifedata.com/resource/pubmed/id/18549623

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-13
pubmed:abstractText
The occurrence and development of Multiple myeloma (MM) are closely related to bone marrow microenvironment in which many signal pathways were involved. Notch signal plays an important role in regulating hematopoietic cells proliferation and differentiation in hemopoietic microenvironment, especially Notch1 has close touch with the occurrence and development of neoplastic hematologic disorders. MM deeply depends on NF-kappaB, and the latter has something to do with oncogenesis, metastasis, proliferation, apoptosis and drug resistance of tumor cells. NF-kappaB2 family has been confirmed as one of target genes of Notch signal. Bortezomib as representative of proteasome inhibitors has been licensed by FDA in treating refractory and relapsing MM, but the mechanism of bortezomib inducing cells apoptosis is not yet clear. This study was purposed to investigate the effects of bortezomib inducing cell apoptosis and influencing the erpression of Notch1 and NF-kappaB in MM RPMI 8226 cells. The effect of bortezomib on apoptosis of MM RPMI8226 cells was assayed by transmission electron microscopy, flow cytometry and TUNEL; the expressions of Notch 1 and NF-kappaB in apoptotic RPMI8226 cells were detected by RT-PCR and immunofluorescence cytochemical staining respectively. The results showed that Notch1 and NF-kappaB highly expressed in RPMI 8226 cells. With the increase of bortezomib concentration, the typical apoptosis features of RPMI8226 cells were observed, the expressions of Notch1 and the expression of NF-kappaB decreased in nuclei and increased in cytoplasm. These changes had significant difference from control after concentration of bortezomib was raised to a certain degree. It is concluded that Notch1 and NF-kappaB signaling pathways participate in bortezomib-inducing RPMI8226 cell apoptosis and there may be some correlation between the Notch 1 and NF-kappaB signaling pathways, indicating that Notch 1 signal may be a latent target in treating MM. This study provides a certain experimental basis for research and development of new drugs.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1009-2137
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
531-7
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
[Proteasome inhibitor induces apoptosis and influences the expression of Notch1 and NF-kappaB in multiple myeloma RPMI8226 cells].
pubmed:affiliation
Department of Hematology, The First Hospital, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't