Linked Life Data

18548154

Source:http://linkedlifedata.com/resource/pubmed/id/18548154

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
2008-6-12
pubmed:abstractText
Fluorescence correlation spectroscopy (FCS) is a powerful tool to measure useful physical quantities such as concentrations, diffusion coefficients, diffusion modes or binding parameters, both in model and cell membranes. However, it can suffer from severe artifacts, especially in non-ideal systems. Here we assess the potential and limitations of standard confocal FCS on lipid membranes and present recent developments which facilitate accurate and quantitative measurements on such systems. In particular, we discuss calibration-free diffusion and concentration measurements using z-scan FCS and two focus FCS and present several approaches using scanning FCS to accurately measure slow dynamics. We also show how surface confined FCS enables the study of membrane dynamics even in presence of a strong cytosolic background and how FCS with a variable detection area can reveal submicroscopic heterogeneities in cell membranes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1463-9076
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3487-97
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
New concepts for fluorescence correlation spectroscopy on membranes.
pubmed:affiliation
Biotechnologisches Zentrum, TU Dresden, Tatzberg 47-51, Dresden, D-01307, Germany.
pubmed:publicationType
Journal Article