Linked Life Data

18544918

Source:http://linkedlifedata.com/resource/pubmed/id/18544918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-6-11
pubmed:abstractText
We aimed to directly align a chromosomal CGH (cCGH) pattern with the gene mapping data by taking advantage of the clustering of the GGCC motif at certain positions in the human genome. The alignment of chromosomal with sequence data was achieved by superimposition of (i) the fluorescence intensity of the sequence specific fluorochrome, Chromomycin A3 (CMA3), (ii) the cCGH fluorescence intensity profile of individual chromosomes and (iii) the GGCC density profile extracted from the Ensembl genome sequence database. The superimposition of these three pieces of information allowed us to precisely localize regions of amplification in the neuroblastoma cell line STA-NB-15. Two prominent cCGH peaks were noted, one at 2p24.3, the position 15.4 mega base (Mb), and the other at 2p23.2, 29.51 Mb. FISH and high resolution array CGH (aCGH) experiments disclosed an amplification of MYCN (16 Mb) and ALK (29.2-29.9 Mb), thus confirming the cCGH data. The combined visualization of sequence information and cCGH data drastically improves the resolution of the method to less than 2 Mb.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1424-859X
pubmed:author
pubmed:copyrightInfo
(c) 2008 S. Karger AG, Basel.
pubmed:issnType
Electronic
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:18544918-Base Sequence, pubmed-meshheading:18544918-Cell Line, Tumor, pubmed-meshheading:18544918-Chromomycin A3, pubmed-meshheading:18544918-Chromosome Mapping, pubmed-meshheading:18544918-Cytogenetics, pubmed-meshheading:18544918-DNA, pubmed-meshheading:18544918-DNA, Neoplasm, pubmed-meshheading:18544918-Fluorescent Dyes, pubmed-meshheading:18544918-Genome, Human, pubmed-meshheading:18544918-Humans, pubmed-meshheading:18544918-In Situ Hybridization, Fluorescence, pubmed-meshheading:18544918-Nuclear Proteins, pubmed-meshheading:18544918-Nucleic Acid Hybridization, pubmed-meshheading:18544918-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:18544918-Oncogene Proteins, pubmed-meshheading:18544918-Protein-Tyrosine Kinases, pubmed-meshheading:18544918-Receptor Protein-Tyrosine Kinases
pubmed:year
2008
pubmed:articleTitle
Sequence based high resolution chromosomal CGH.
pubmed:affiliation
CCRI, Children's Cancer Research Institute, St. Anna Kinderkrebsforschung, Vienna, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't