Linked Life Data

18542869

Source:http://linkedlifedata.com/resource/pubmed/id/18542869

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-6-10
pubmed:abstractText
Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins that can be measured by NMR spectroscopy in the presence of all the unlabeled proteins in the reaction mixture. No chromatographic purification is required. Using an extract from Escherichia coli in a simple dialysis system, the target protein can be prepared at a typical concentration of about 1 mg/ml, which is sufficient for subsequent analysis by NMR. This chapter describes in detail the protocol used in the authors' laboratory.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
426
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-68
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Cell-free protein synthesis for analysis by NMR spectroscopy.
pubmed:affiliation
Research School of Chemistry, Australian National University, Canberra, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't