|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2008-7-8
|
|
pubmed:abstractText |
Immunoglobulin heavy chain (IgH) class switch recombination (CSR) replaces the initially expressed IgH Cmu exons with a set of downstream IgH constant region (C(H)) exons. Individual sets of C(H) exons are flanked upstream by long (1-10-kb) repetitive switch (S) regions, with CSR involving a deletional recombination event between the donor Smu region and a downstream S region. Targeting CSR to specific S regions might be mediated by S region-specific factors. To test the role of endogenous S region sequences in targeting specific CSR events, we generated mutant B cells in which the endogenous 10-kb Sgamma1 region was replaced with wild-type (WT) or synthetic 2-kb Sgamma3 sequences or a synthetic 2-kb Sgamma1 sequence. We found that both the inserted endogenous and synthetic Sgamma3 sequences functioned similarly to a size-matched synthetic Sgamma1 sequence to mediate substantial CSR to IgG1 in mutant B cells activated under conditions that stimulate IgG1 switching in WT B cells. We conclude that Sgamma3 can function similarly to Sgamma1 in mediating endogenous CSR to IgG1. The approach that we have developed will facilitate assays for IgH isotype-specific functions of other endogenous S regions.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-10438941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-10770803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-11007474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-11148220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-11254712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-11801452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-11884453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-12679811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-12679812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-14962903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-14993249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-15273694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-15531884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-15684074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-16818776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-17109470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-17170253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-17328676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-17560275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-17713479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-18370922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-2987353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-3007121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-3028778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-8441648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-8506294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-8598461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-9317110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18541713-9881975
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1540-9538
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
7
|
|
pubmed:volume |
205
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1567-72
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18541713-Animals,
pubmed-meshheading:18541713-B-Lymphocytes,
pubmed-meshheading:18541713-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:18541713-Immunoglobulin Constant Regions,
pubmed-meshheading:18541713-Immunoglobulin Heavy Chains,
pubmed-meshheading:18541713-Mice,
pubmed-meshheading:18541713-Mice, Transgenic,
pubmed-meshheading:18541713-Mutation,
pubmed-meshheading:18541713-Recombination, Genetic
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Sgamma3 switch sequences function in place of endogenous Sgamma1 to mediate antibody class switching.
|
|
pubmed:affiliation |
Department of Genetics, Howard Hughes Medical Institute, Children's Hospital, Immune Disease Institute, Harvard University Medical School, Boston, MA 02115, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
